Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster

被引：19

作者：

Sharma, Anurag ^{[1
,3
]}

Mishra, M. ^{[1
,3
]}

Ram, K. Ravi ^{[1
,3
]}

Kumar, R. ^{[2
,3
]}

Abdin, M. Z. ^{[4
]}

Chowdhuri, D. Kar ^{[1
,3
]}

机构：

[1] Indian Inst Toxicol Res, Embryotoxicol Sect, Lucknow 226001, Uttar Pradesh, India

[2] Indian Inst Toxicol Res, Analyt Chem Sect, Lucknow 226001, Uttar Pradesh, India

[3] CSIR, New Delhi 110001, India

[4] Jamia Hamdard, Dept Biotechnol, New Delhi 110062, India

来源：

CHEMOSPHERE | 2011年 / 82卷 / 03期

关键词：

Endosulfan; Drosophila melanogaster; Gene expression; Stress response; Organismal effect; OXIDATIVE STRESS; GENE-EXPRESSION; HSP70; INDUCTION; RESPONSES; ANNOTATION; RESISTANCE; PROFILES; PROTEINS; EXPOSURE;

D O I：

10.1016/j.chemosphere.2010.10.002

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Indiscriminate use of agrochemicals worldwide, particularly, persistent organic pollutants (POPs), is of concern. Endosulfan, a POP, is used by various developing/developed nations and is known to adversely affect the development and the hormonal profiles of humans and animals. However, little is known about the molecular players/pathways underlying the adverse effects of endosulfan. We therefore analyzed the global gene expression changes and subsequent adverse effects of endosulfan using Drosophila. We used Drosophila melanogaster keeping in view of its well annotated genome and the wealth of genetic/molecular reagents available for this model organism. We exposed third instar larvae of D. melanogaster to endosulfan (2.0 mu g mL(-1)) for 24 h and using microarray, we identified differential expression of 256 genes in exposed organisms compared to controls. These genes are associated with cellular processes such as development, stress and immune response and metabolism. Microarray results were validated through quantitative PCR and biochemical assay on a subset of genes/proteins. Taking cues from microarray data, we analyzed the effect of endosulfan on development, emergence and survival of the organism. In exposed organisms, we observed deformities in hind-legs, reminiscent of those observed in higher organisms exposed to endosulfan. In addition, we observed delayed and/or reduced emergence in exposed organisms when compared to their respective controls. Together, our studies not only highlight the adverse effects of endosulfan on the organism but also provide an insight into the possible genetic perturbations underlying these effects, which might have potential implications to higher organisms. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：370 / 376

页数：7