Inactivation and sublethal injury of Escherichia coli O157:H7 cells induced by heat in citrate phosphate buffer and apple juice (both at pH 3.8) were studied, and the effect of a combined preservation treatment using citral and heat treatments was determined. Heat resistance of E. coli O157:H7 was similar in both treatment media; after 27 min at 54 degrees C, 3 log units of the initial cell population was inactivated in both treatment media. However, under less harsh conditions a protective effect of apple juice was found. Whereas inactivation followed linear kinetics in the citrate phosphate buffer, when cells were treated in apple juice the survival curves were concave downward. Heat treatment caused a great degree of sublethal injury; 4 min at 54 degrees C inactivated less than 0.5 log CFU/ml but sublethally injured more than 3 log CFU/ml. The addition of 18 and 200 ppm of citral to the treatment medium acted synergistically with heat at 54 degrees C to inactivate 3 x 10(4) and 3 x 10(7) CFU/ml, respectively. Addition of citral thus reduced the time needed to inactivate 1 log unit of the initial E. coli O157:H7 population from 8.9 to 1.7 min. These results indicate that a combined process of heat and citral can inactivate E. coli O157:H7 cells and reduce their potential negative effects.
机构:
Cornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USACornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USA
Usaga, Jessie
Worobo, Randy W.
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Cornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USACornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USA
Worobo, Randy W.
Padilla-Zakour, Olga I.
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Cornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USACornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USA
机构:
Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USAUniv Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA
Lim, Ji Youn
Yoon, Jang W.
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Konkuk Univ, Sch Med, Adv Human Resource & Res Grp Med Sci BK21, Seoul 143701, South KoreaUniv Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA
Yoon, Jang W.
Hovde, Carolyn J.
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Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USAUniv Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA
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Univ Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, MalaysiaUniv Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, Malaysia
Kiang, W. -S.
Bhat, R.
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Univ Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, MalaysiaUniv Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, Malaysia
Bhat, R.
Rosma, A.
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Univ Sains Malaysia, Sch Ind Technol, Bioproc Technol Div, Minden 11800, Penang, MalaysiaUniv Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, Malaysia
Rosma, A.
Cheng, L. -H.
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Univ Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, MalaysiaUniv Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, Malaysia
机构:
Cornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USACornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USA
Usaga, Jessie
Worobo, Randy W.
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Cornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USACornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USA
Worobo, Randy W.
Padilla-Zakour, Olga I.
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Cornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USACornell Univ, New York State Agr Expt Stn, Dept Food Sci, Geneva, NY 14456 USA
机构:
Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USAUniv Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA
Lim, Ji Youn
Yoon, Jang W.
论文数: 0引用数: 0
h-index: 0
机构:
Konkuk Univ, Sch Med, Adv Human Resource & Res Grp Med Sci BK21, Seoul 143701, South KoreaUniv Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA
Yoon, Jang W.
Hovde, Carolyn J.
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Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USAUniv Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA
机构:
Univ Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, MalaysiaUniv Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, Malaysia
Kiang, W. -S.
Bhat, R.
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机构:
Univ Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, MalaysiaUniv Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, Malaysia
Bhat, R.
Rosma, A.
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h-index: 0
机构:
Univ Sains Malaysia, Sch Ind Technol, Bioproc Technol Div, Minden 11800, Penang, MalaysiaUniv Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, Malaysia
Rosma, A.
Cheng, L. -H.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, MalaysiaUniv Sains Malaysia, Sch Ind Technol, Food Technol Div, Minden 11800, Penang, Malaysia