Glutamate co-transmission from developing medial nucleus of the trapezoid body - Lateral superior olive synapses is cochlear dependent in kanamycin-treated rats

被引：11

作者：

Lee, Jae Ho ^{[6
]}

Pradhan, Jonu ^{[5
]}

Maskey, Dhiraj ^{[4
]}

Park, Ki Sup ^{[4
]}

Hong, Sung Hwa ^{[3
]}

Suh, Myung-Whan ^{[2
]}

Kim, Myeung Ju ^{[4
]}

Ahn, Seung Cheol ^{[1
]}

机构：

[1] Dankook Univ, Coll Med, Dept Physiol, Cheonan Si 330714, Chungnam, South Korea

[2] Dankook Univ, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Cheonan Si 330714, Chungnam, South Korea

[3] Sungkyunkwan Univ, Samsung Med Ctr, Dept Otorhinolaryngol Head & Neck Surg, Sch Med, Seoul 135710, South Korea

[4] Dankook Univ, Coll Med, Dept Anat, Cheonan Si 330714, Chungnam, South Korea

[5] Dankook Univ, Dept Nanobio Med Sci, Cheonan Si 330714, Chungnam, South Korea

[6] Dankook Univ, Inst Tissue Regenerat Engn ITREN, Cheonan Si 330714, Chungnam, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 405卷 / 02期

关键词：

MNTB; LSO; Kanamycin; Glutamate; VGLUT3; Cochlea; STEM AUDITORY NUCLEI; EAR OSSICLE REMOVAL; INFERIOR COLLICULUS; BRAIN-STEM; PLASTIC CHANGES; CIRCLING MOUSE; PROJECTIONS; COMPLEX; GERBIL; RESPONSES;

D O I：

10.1016/j.bbrc.2010.12.129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cochlear dependency of glutamate co-transmission at the medial nucleus of the trapezoid body (MNTB) the lateral superior olive (LSO) synapses was investigated using developing rats treated with high dose kanamycin. Rats were treated with kanamycin from postnatal day (P) 3 to P8. A scanning electron microscopic study on P9 demonstrated partial cochlear hair cell damage. A whole cell voltage clamp experiment demonstrated the increased glutamatergic portion of postsynaptic currents (PSCs) elicited by MNTB stimulation in P9-P11 kanamycin-treated rats. The enhanced VGLUT3 immunoreactivities (IRs) in kanamycin-treated rats and asymmetric VGLUT3 IRs in the LSO of unilaterally cochlear ablated rats supported the electrophysiologic data. Taken together, it is concluded that glutamate co-transmission is cochlear-dependent and enhanced glutamate co-transmission in kanamycin-treated rats is induced by partial cochlear damage. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：162 / 167

页数：6

共 25 条

[1] Developmental pattern of three vesicular glutamate transporters in the rat superior olivary complex [J].

Blaesse, P ;

Ehrhardt, S ;

Friauf, E ;

Nothwang, HG .

CELL AND TISSUE RESEARCH, 2005, 320 (01) :33-50

[2]

Bledsoe SC, 1995, NEUROREPORT, V7, P225, DOI 10.1097/00001756-199512000-00054

[3] Changes in glycine immunoreactivity in the rat superior olivary complex following deafness [J].

Buras, ED ;

Holt, AG ;

Griffith, RD ;

Asako, M ;

Altschuler, RA .

JOURNAL OF COMPARATIVE NEUROLOGY, 2006, 494 (01) :179-189

[4] SUPRA-NORMAL SENSITIVITY TO OTOTOXIC ANTIBIOTIC OF THE DEVELOPING RAT COCHLEA [J].

CARLIER, E ;

PUJOL, R .

ARCHIVES OF OTO-RHINO-LARYNGOLOGY-ARCHIV FUR OHREN-NASEN-UND KEHLKOPFHEILKUNDE, 1980, 226 (03) :129-133

[5] Cochlear pathology of the circling mouse: a new mouse model of DFNB6 [J].

Chung, Won-Ho ;

Kim, Ki Ryung ;

Cho, Yang-Sun ;

Cho, Do-Yeon ;

Woo, Ji Hwan ;

Ryoo, Zae Young ;

Cho, Kyoung In ;

Hong, Sung Hwa .

ACTA OTO-LARYNGOLOGICA, 2007, 127 (03) :244-251

[6] Inhibitory synapses in the developing auditory system are glutamatergic [J].

Gillespie, DC ;

Kim, G ;

Kandler, K .

NATURE NEUROSCIENCE, 2005, 8 (03) :332-338

[7] Glutamatergic Transmission Is Sustained at a Later Period of Development of Medial Nucleus of the Trapezoid Body-Lateral Superior Olive Synapses in Circling Mice [J].

Hong, Sung Hwa ;

Kim, Myeung Ju ;

Ahn, Seung Cheol .

JOURNAL OF NEUROSCIENCE, 2008, 28 (48) :13003-13007

[8] Elimination and strengthening of glycinergic/GABAergic connections during tonotopic map formation [J].

Kim, G ;

Kandler, K .

NATURE NEUROSCIENCE, 2003, 6 (03) :282-290

[9] SINGLE-UNIT RESPONSES IN THE INFERIOR COLLICULUS - EFFECTS OF NEONATAL UNILATERAL COCHLEAR ABLATION [J].

KITZES, LM ;

SEMPLE, MN .

JOURNAL OF NEUROPHYSIOLOGY, 1985, 53 (06) :1483-1500

[10] SOME PHYSIOLOGICAL CONSEQUENCES OF NEONATAL COCHLEAR DESTRUCTION IN THE INFERIOR COLLICULUS OF THE GERBIL, MERIONES-UNGUICULATUS [J].

KITZES, LM .

BRAIN RESEARCH, 1984, 306 (1-2) :171-178

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