Variation in the levels of the different xylanase inhibitors in grain and flour of 20 French wheat cultivars

A xylanase from Aspergillus aculeatus (glycoside hydrolase family 10). uninhibited by TAXI, and a xylanase from Bacillus subtilis (family 11), uninhibited by XIP-I, were selected to quantify the respective apparent levels of XIP-I and TAXI inhibitors, in flours and grains of 20 wheat varieties. The apparent TAXI amount ranged from 0.05 to 0.19 mg/g in flour (mean: 0.11 mg/g) and from 0.07 to 0.2 mg/g in grain (mean: 0.14 mg/g). The range observed for XIP-I was 0.12-0.6 mg/g in flour (mean 0.32 mg/g), and 0.21-0.56 mg/g in grain (mean: 0.41 mg/g). The inhibition profile of the xylanase from A. aculeatus by a crude inhibitor preparation suggested the presence of an additional component in wheat flour, responsible for an increase in inhibition. (c) 2004 Elsevier Ltd. All rights reserved.