To solve the nitrate pollution of groundwater, it is necessary to research the remediation technology of nitrate pollution of groundwater. For the current remediation technology to remedy nitrate contaminated groundwater, there are problems of poor degradation efficiency, low degradation efficiency, and poor removal efficiency. To address these problems, a rapid remediation of nitrate pollution of groundwater is proposed in this paper. A laboratory test equipment of double-chambered non-bioactive steel fuel cell (SFC) and microbial fuel cell (MFC) were constructed. The influence of the selection of cathode material, the difference of pH in the cathode chamber, the difference of nitrate concentration, and the addition of microorganism in the cathode chamber on the removal of nitrate in groundwater was researched. Experimental results show that the SFC/MFC equipment can effectively promote denitrification of nitrate. The removal efficiencies of SFC and MFC equipment are 62.1% and 67.05%, respectively. The average degradation rate of nitrate is about 6.59 mgNO(3)(-)/L.d and 7.12 mg NO3-/L.d, respectively. Stainless steel plate with PbO2 cathode is more conducive to nitrate degradation than stainless steel plates without coating and carbon cloth. Near neutral and moderately concentrated nitrate solutions are more conducive to nitrate degradation. The addition of microorganisms in the cathode chamber can greatly improve the nitrate degradation rate. The degradation rate of nitrate in the cathode chamber of SFC/MFC laboratory test equipment is related to the output voltage of SFC/MFC. The higher the output voltage, the faster the nitrate degradation rate. It is verified that the proposed technology has good degradation effect, high degradation efficiency, and good removal efficiency.