Activity of Disinfecting Biocides and Enzymes of Proteases and Amylases on Bacteria in Biofilms

The presence of microbial biofilms on the surfaces of medical instruments, operating equipment, prostheses, catheters, technological lines in the food industry is a fact that contributes to the infection of the macroorganism and contamination of raw materials and products. The aim of the work was to investigate the effect of disinfecting substances Vantocilu TG and Catamine AB and their combination with enzymes on bacteria in biofilms. In the experiments, we used disinfecting substances Vantocil TG (Arch Biocides LTD, Great Britain) and Catamine AB (Intersintez, Ukraine). Enzymes: Everlase 16 L and Termamyl 300 L (Novozymes, Denmark). It was found that bacteria in biofilms withstood the minimum bactericidal concentration of Vantocil and Catamine, which was set on their planktonic forms. From one mL of wash from the biofilm after exposure to Vantocil were isolated from 1.9x10(3) to 4.3x10(3) microbial cells, and after treatment with Catamine from 5.6x10(3) to 1.7x10(4). At the same time, after treatment of biofilms with Vantocil and Catamine together with enzymes, a decrease in the number of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa cells was observed, on average by two orders up to 101 CFU/mL, compared with treatment with biocides only. That is, there is a clear synergy of enzymes and biocides, which ultimately has a more detrimental effect on bacteria in biofilms.