microRNA-16 Is Downregulated During Insulin Resistance and Controls Skeletal Muscle Protein Accretion

被引：57

作者：

Lee, David E. ^{[1
]}

Brown, Jacob L. ^{[1
]}

Rosa, Megan E. ^{[1
]}

Brown, Lemuel A. ^{[2
]}

Perry, Richard A., Jr. ^{[2
]}

Wiggs, Michael P. ^{[3
]}

Nilsson, Mats I. ^{[3
]}

Crouse, Stephen F. ^{[4
]}

Fluckey, James D. ^{[3
]}

Washington, Tyrone A. ^{[2
]}

Greene, Nicholas P. ^{[1
,3
,4
]}

机构：

[1] Univ Arkansas, Dept Hlth Human Performance & Recreat, Human Performance Lab, Integrat Muscle Metab Lab, 155 Stadium Dr,321 HPER Bldg, Fayetteville, AR 72701 USA

[2] Univ Arkansas, Dept Hlth Human Performance & Recreat, Human Performance Lab, Exercise Muscle Biol Lab, Fayetteville, AR 72701 USA

[3] Texas A&M Univ, Dept Hlth & Kinesiol, Muscle Biol Lab, College Stn, TX 77843 USA

[4] Texas A&M Univ, Dept Hlth & Kinesiol, Appl Exercise Sci Lab, College Stn, TX 77843 USA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2016年 / 117卷 / 08期

关键词：

PROTEIN SYNTHESIS; AUTOPHAGY; SUnSET; TYPE II DIABETES MELLITUS; MITOCHONDRIAL BIOGENESIS; EXERCISE; EXPRESSION; AUTOPHAGY; TARGET; PROLIFERATION; PGC-1-ALPHA; ATROPHY;

D O I：

10.1002/jcb.25476

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin resistant diabetes, currently at epidemic levels in developed countries, begins in the skeletal muscle and is linked to altered protein turnover. microRNAs downregulate targeted mRNA translation decreasing the amount of translated protein, thereby regulating many cellular processes. Regulation of miRNAs and their function in skeletal muscle insulin resistance is largely unexplored. The purpose of this study was to identify the effects of insulin resistance on contents of skeletal muscle miRNAs with potential functions in protein turnover. We examined miRs -1, -16, -23, -27, -133a, -133b, and -206 in muscles of Zucker rats. miR-1 was 5- to 10-fold greater in obesity, whereas miRs-16 and -133b were repressed approximate to 50% in obese compared to lean rats, with no other alterations in miRNA contents. miR-16 correlated to protein synthesis in lean, but not obese rats. miR-16 reduction by lipid overload was verified in-vivo by diet-induced obesity and in-vitro using a diacylglycerol analog. A role for miR-16 in protein turnover of skeletal myocytes was established using transient overexpression and anti-miR inhibition. miR-16 overexpression resulted in lower protein synthesis (puromycin incorporation, approximate to 25-50%), mTOR (approximate to 25%), and p70S6K1 (approximate to 40%) in starved and insulin stimulated myoblasts. Conversely, anti-miR-16 increased basal protein synthesis (puromycin incorporation, approximate to 75%), mTOR (approximate to 100%), and p70S6K1 (approximate to 100%). Autophagy was enhanced by miR-16 overexpression (approximate to 50% less BCL-2, approximate to 100% greater LC3II/I, approximate to 50% less p62) and impaired with miR-16 inhibition (approximate to 45% greater BCL-2, approximate to 25% less total LC3, approximate to 50% greater p62). This study demonstrates reduced miR-16 during insulin resistance and establishes miR-16 control of protein accretion in skeletal muscle. J. Cell. Biochem. 117: 1775-1787, 2016. (c) 2015 Wiley Periodicals, Inc.

引用

页码：1775 / 1787

页数：13

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