Cloning and sequencing of a region of Vibrio cholerae 0139 Bengal and its use in PCR-based detection

被引：6
作者：
Falklind, S
Stark, M
Albert, MJ
Uhlen, M
Lundeberg, J
Weintraub, A
机构：
[1] ROYAL INST TECHNOL, DEPT BIOCHEM & BIOTECHNOL, KTH, S-10044 STOCKHOLM, SWEDEN
[2] HUDDINGE UNIV HOSP, KAROLINSKA INST, DEPT IMMUNOL MICROBIOL PATHOL & INFECT DIS, DIV CLIN BACTERIOL, S-14186 HUDDINGE, SWEDEN
[3] INT CTR DIARRHOEAL DIS RES, DHAKA, BANGLADESH
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 12期
关键词：
D O I：
10.1128/JCM.34.12.2904-2908.1996
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region could be used for the specific detection of V. cholerae O139 Bengal, a PCR system was established. The specificity and sensitivity of the PCR were investigated by analyzing 240 strains within the family Vibrionaceae and 178 strains of other gram-negative bacteria. All V. cholerae O139 Bengal strains tested were positive, and none of the 384 control strains were amplified. The sensitivity of the assay was 10(2) CFU/ml.
引用
收藏
页码：2904 / 2908
页数：5
相关论文
共 35 条
[1]   VIBRIO-CHOLERAE O139 BENGAL [J].
ALBERT, MJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (10) :2345-2349
[2]  
ALBERT MJ, 1993, LANCET, V342, P387, DOI 10.1016/0140-6736(93)92811-7
[3]  
ALBERT MJ, 1993, LANCET, V341, P704
[4]   LACK OF CROSS-PROTECTION AGAINST DIARRHEA DUE TO VIBRIO-CHOLERAE-O139 (BENGAL STRAIN) AFTER ORAL IMMUNIZATION OF RABBITS WITH V-CHOLERAE-O1 VACCINE STRAIN CVD103-HGR [J].
ALBERT, MJ ;
ALAM, K ;
ANSARUZZAMAN, M ;
QADRI, F ;
SACK, RB .
JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (01) :230-231
[5]  
ALBERT MJ, 1993, J DIARRHOEAL DIS RES, V11, P207
[6]   Cloning and sequence of a region encoding a surface polysaccharide of Vibrio cholerae O139 and characterization of the insertion site in the chromosome of Vibrio cholerae O1 [J].
Comstock, LE ;
Johnson, JA ;
Michalski, JM ;
Morris, JG ;
Kaper, JB .
MOLECULAR MICROBIOLOGY, 1996, 19 (04) :815-826
[7]   THE CAPSULE AND O-ANTIGEN IN VIBRIO-CHOLERAE-O139 BENGAL ARE ASSOCIATED WITH A GENETIC REGION NOT PRESENT IN VIBRIO-CHOLERAE-O1 [J].
COMSTOCK, LE ;
MANEVAL, D ;
PANIGRAHI, P ;
JOSEPH, A ;
LEVINE, MM ;
KAPER, JB ;
MORRIS, JG ;
JOHNSON, JA .
INFECTION AND IMMUNITY, 1995, 63 (01) :317-323
[8]  
EKLIND K, 1978, P 9 INT S CARB CHEM, P493
[9]  
GORSHKOVA RP, 1983, BIOORG KHIM+, V9, P1068
[10]   STRUCTURAL STUDIES ON O-SPECIFIC SIDE-CHAINS OF CELL-WALL LIPOPOLYSACCHARIDE FROM SALMONELLA TYPHIMURIUM 395 MS [J].
HELLERQVIST, CG ;
LINDBERG, B ;
SVENSSON, S ;
HOLME, T ;
LINDBERG, AA .
CARBOHYDRATE RESEARCH, 1968, 8 (01) :43-+
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