Whole blood flow cytometry protocol for the assessment of platelet phenotype, function, and cellular interactions

被引:10
作者
Yaw, Hui Ping [1 ,2 ]
van den Helm, Suelyn [1 ]
Linden, Matthew [3 ]
Monagle, Paul [1 ,2 ,4 ]
Ignjatovic, Vera [1 ,2 ]
机构
[1] Murdoch Childrens Res Inst, Haematol Res, Melbourne, Vic, Australia
[2] Univ Melbourne, Dept Paediat, Melbourne, Vic, Australia
[3] Univ Western Australia, Sch Biomed Sci, Perth, WA, Australia
[4] Royal Childrens Hosp, Dept Clin Haematol, Melbourne, Vic, Australia
基金
英国医学研究理事会;
关键词
Flow cytometry; platelet function; platelet-leukocyte aggregates; whole blood; FUNCTION TESTS; FLUORESCENCE; ACTIVATION; THROMBIN; STORAGE;
D O I
10.1080/09537104.2020.1810222
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Platelets are a key component of the hemostatic system and their roles in inflammation via interactions with leukocytes have also gained attention in recent years. Changes in platelet phenotype and function can cause bleeding and/or thrombosis and, as such, monitoring platelet-specific changes is crucial to assessing hemostasis in the clinical setting. Currently, available platelet function tests such as platelet aggregometry and thromboelastography require a large volume of blood, which is a major limitation for the pediatric population. Whole blood flow cytometric analysis of platelets is increasingly utilized in recent years, primarily due to the sensitivity of this method, but also because it only requires a small amount of blood with minimal sample manipulation. We have developed a whole blood flow cytometry methodological approach that enables the assessment of platelet phenotype, function, and their interactions with monocytes and neutrophils.
引用
收藏
页码:786 / 793
页数:8
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