Field portable and autonomous immunosensors for the detection of environmental contaminants

被引:0
作者
Blake, DA [1 ]
Yu, HN
James, EA
Li, X
Blake, RC
机构
[1] Tulane Univ, Hlth Sci Ctr, Dept Biochem, 1430 Tulane Ave SL-43, New Orleans, LA 70112 USA
[2] Xavier Univ Louisiana, Sch Pharmacy, New Oleans, LA USA
来源
URANIUM IN THE ENVIRONMENT: MINING IMPACT AND CONSEQUENCES | 2006年
关键词
D O I
10.1007/3-540-28367-6_8
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A monoclonal antibody that binds with high affinity to UO22+ complexed with the chelator 2,9-dicarboxyl-1,10-phenanthroline was used to develop a sensor-based assay for UO22+. The assay range for UO22+ was 0.5 to 25 nM (0.12 to 6 ppb). The average coefficients of variation in the assay was 2.2%. The immunoassay results were comparable to those obtained using the Kinetic Phosphorescence Assay. The versatility of this sensor platform was further demonstrated by the development of an immunoassay for caffeine.
引用
收藏
页码:87 / +
页数:3
相关论文
共 13 条
[1]   Determination of carbaryl, carbofuran and methiocarb in cucumbers and strawberries by monoclonal enzyme immunoassays and high-performance liquid chromatography with fluorescence detection -: An analytical comparison [J].
Abad, A ;
Moreno, MJ ;
Pelegrí, R ;
Martínez, MI ;
Sáez, A ;
Gamón, M ;
Montoya, A .
JOURNAL OF CHROMATOGRAPHY A, 1999, 833 (01) :3-12
[2]   Novel monoclonal antibodies with specificity for chelated uranium(VI): Isolation and binding properties [J].
Blake, RC ;
Pavlov, AR ;
Khosraviani, M ;
Ensley, HE ;
Kiefer, GE ;
Yu, H ;
Li, X ;
Blake, DA .
BIOCONJUGATE CHEMISTRY, 2004, 15 (05) :1125-1136
[3]  
BLAKE RC, 2003, METHODS MOL BIOL ANT, P417
[4]   IMMUNOCHEMICAL METHODS FOR ENVIRONMENTAL MONITORING [J].
GIRAUDI, G ;
BAGGIANI, C .
NUCLEAR MEDICINE AND BIOLOGY, 1994, 21 (03) :557-572
[5]  
GLASSMEYER SI, 2003, US EPAS RES MICR DRI
[6]   Pharmaceuticals, hormones, and other organic wastewater contaminants in US streams, 1999-2000: A national reconnaissance [J].
Kolpin, DW ;
Furlong, ET ;
Meyer, MT ;
Thurman, EM ;
Zaugg, SD ;
Barber, LB ;
Buxton, HT .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (06) :1202-1211
[7]  
Moore J. W., 1981, KINETICS MECH, V3rd, P60
[8]   Molecular tracers of organic matter sources to surface water resources [J].
Standley, LJ ;
Kaplan, LA ;
Smith, D .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2000, 34 (15) :3124-3130
[9]   Rapid assays for environmental and biological monitoring [J].
Szurdoki, F ;
Jaeger, L ;
Harris, A ;
Kido, H ;
Wengatz, I ;
Goodrow, MH ;
Szekacs, A ;
Wortberg, M ;
Zheng, J ;
Stoutamire, DW ;
Sanborn, JR ;
Gilman, SD ;
Jones, AD ;
Gee, SJ ;
Choudary, PV ;
Hammock, BD .
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 1996, 31 (03) :451-458
[10]   Bioseparation and bioanalytical techniques in environmental monitoring [J].
Van Emon, JM ;
Gerlach, CL ;
Bowman, K .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1998, 715 (01) :211-228