Sonic hedgehog enhances the proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells

被引：43
作者：
Cai, Jia-Qin [2 ,3 ]
Huang, Yi-Zhou [2 ]
Chen, Xiao-He [2 ]
Xie, Hong-Lei [2 ]
Zhu, Hong-Ming [2 ]
Tang, Li [2 ,3 ]
Yang, Zhi-Ming [2 ]
Huang, Yong-Can [1 ]
Deng, Li [2 ]
机构：
[1] Univ Hong Kong, Li Ka Shing Fac Med, Dept Orthopaed & Traumatol, Hong Kong 999077, Hong Kong, Peoples R China
[2] Sichuan Univ, W China Hosp, Regenerat Med Res Ctr, Div Stem Cell & Tissue Engn, Chengdu 610041, Peoples R China
[3] Sichuan Univ, W China Sch Pharm, Chengdu 610041, Peoples R China
来源：
CELL BIOLOGY INTERNATIONAL | 2012年 / 36卷 / 04期
基金：
中国国家自然科学基金;
关键词：
bone marrow; mesenchymal stem cell (MSC); osteogenic differentiation; proliferation; sonic hedgehog; OSTEOBLAST DIFFERENTIATION; INDIAN HEDGEHOG; EXPRESSION; CHONDROCYTES; MUTATIONS; INCREASES; PROTEINS; LINEAGE; IHH;
D O I：
10.1042/CBI20110284
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
MSCs (mesenchymal stem cells) may be promising seed cells for tissue regeneration because of their self-renewal and multi-differentiation potential. Shh (sonic hedgehog) is involved in the skeletal formation during embryo development and skeletal regeneration. However, how Shh regulates the biological characteristics of BM-MSCs (bone marrow-derived MSCs) is poorly understood. We have investigated the effect of rShh-N (recombinant N-terminal Shh) on the proliferation and osteogenic differentiation of rBM-MSCs (rat BM-MSCs) in vitro. rBM-MSCs were treated with rShh-N at concentrations up to 200 ng/ml. Proliferation and colony-forming ability of rBM-MSCs were increased in a dose-dependent manner. rShh-N increased the ratio of cells in S and G(2)/M phase, as well as the number of Ki-67(+) cells. In addition, ALP (alkaline phosphatase) activity and matrix mineralization were enhanced by 200 ng/ml rShh-N. Real-time PCR showed that rShh-N (200 ng/ml) up-regulated the expression of genes encoding Cbfa-1 (core-binding factor alpha 1), osteocalcin, ALP and collagen type I in rBM-MSCs. This information reveals some potential of rShh-N in the therapeutics of bone-related diseases.
引用
收藏
页码：349 / 355
页数：7
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