Survival of infectious prions in water

被引：13

作者：

Miles, Syreeta L. ^{[1
]}

Takizawa, Kazue ^{[1
]}

Gerba, Charles P. ^{[1
]}

Pepper, Ian L. ^{[1
]}

机构：

[1] Univ Arizona, Environm Res Lab, Soil Water & Environm Sci Dept, Tucson, AZ 85756 USA

来源：

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING | 2011年 / 46卷 / 09期

基金：

美国国家科学基金会;

关键词：

Prions; infectivity; water; wastewater; survival; temperature; PROTEIN PRPSC; FATE; DEGRADATION; BSE;

D O I：

10.1080/10934529.2011.586247

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The objective of this study was to evaluate the fate of infectious prions in water. Known concentrations of infectious prions were added to deionized water, tap water, and wastewater. Samples were incubated at 25 degrees C, 37 degrees C, and 50 degrees C for 1 to 8 weeks. The standard scrapie cell assay (SSCA) which includes the ELISPOT (Enzyme Linked Immuno-Spot) reaction was performed to determine prion infectivity and quantity as a function of time. A reduction of infectious prions was observed at 25 degrees C, 37 degrees C, and 50 degrees C ranging between 0.5-log(10) and 1.4-log(10) in one week. Results suggest that organic matter was instrumental in protecting infectious prions, allowing them to remain infectious for a longer period of time. Thus, our data effectively show a quantifiable reduction of infectious prions in water and identifies some of the components that may influence infectivity.

引用

页码：938 / 943

页数：6

共 21 条

[1]

Baird R.B., 2005, Standard methods for the examination of water and wastewater

[2] Transmissions to mice indicate that 'new variant' CJD is caused by the BSE agent [J].

Bruce, ME ;

Will, RG ;

Ironside, JW ;

McConnell, I ;

Drummond, D ;

Suttie, A ;

McCardle, L ;

Chree, A ;

Hope, J ;

Birkett, C ;

Cousens, S ;

Fraser, H ;

Bostock, CJ .

NATURE, 1997, 389 (6650) :498-501

[3]

CHANDLER RL, 1961, LANCET, V1, P1378

[4] Detection and survival of prion agents in aquatic environments [J].

de Motes, C. Maluquer ;

Cano, M. J. ;

Torres, J. M. ;

Pumarola, M. ;

Girones, R. .

WATER RESEARCH, 2008, 42 (10-11) :2465-2472

[5] Development of a risk assessment for BSE in the aquatic environment [J].

Gale, P ;

Young, C ;

Stanfield, G ;

Oakes, D .

JOURNAL OF APPLIED MICROBIOLOGY, 1998, 84 (04) :467-477

[6]

Geldreich E., 1996, Microbial Quality of Water Supply in Distribution Systems

[7] Detection of infectious prions in urine [J].

Gonzalez-Romero, Dennisse ;

Barria, Marcelo A. ;

Leon, Patricia ;

Morales, Rodrigo ;

Soto, Claudio .

FEBS LETTERS, 2008, 582 (21-22) :3161-3166

[8] Persistence of pathogenic prion protein during simulated wastewater treatment processes [J].

Hinckley, Glen T. ;

Johnson, Christopher J. ;

Jacobson, Kurt H. ;

Bartholomay, Christian ;

Mcmahon, Katherine D. ;

Mckenzie, Debbie ;

Aiken, Judd M. ;

Pedersen, Joel A. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2008, 42 (14) :5254-5259

[9] Prion protein: detection in 'spiked' anaerobic sludge and degradation experiments under anaerobic conditions [J].

Kirchmayr, R. ;

Reichl, H. E. ;

Schildorfer, H. ;

Braun, R. ;

Somerville, R. A. .

WATER SCIENCE AND TECHNOLOGY, 2006, 53 (08) :91-98

[10]

Mahal Sukhvir P., 2008, V459, P49, DOI 10.1007/978-1-59745-234-2_4

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