Role of guanylate binding protein-1 in vascular defects associated with chronic inflammatory diseases

被引:25
作者
Hammon, Matthias [2 ]
Herrmann, Martin [3 ]
Bleiziffer, Oliver [2 ]
Pryymachuk, Galyna [2 ]
Andreoli, Laura [4 ]
Munoz, Luis E. [3 ]
Amann, Kerstin U. [5 ]
Mondini, Michele [6 ]
Gariglio, Marisa [6 ]
Airo, Paolo [4 ]
Schellerer, Vera S.
Hatzopoulos, Antonis K. [7 ]
Horch, Raymund E. [2 ]
Kneser, Ulrich [2 ]
Stuerzl, Michael [1 ]
Naschberger, Elisabeth [1 ]
机构
[1] Univ Med Ctr Erlangen, Dept Surg, Div Mol & Expt Surg, D-91054 Erlangen, Germany
[2] Univ Med Ctr Erlangen, Dept Plast & Hand Surg, D-91054 Erlangen, Germany
[3] Univ Med Ctr Erlangen, Dept Internal Med 3, Inst Clin Immunol & Rheumatol, D-91054 Erlangen, Germany
[4] Univ Clin Brescia, Brescia, Italy
[5] Univ Med Ctr Erlangen, Inst Pathol, Div Nephropathol, D-91054 Erlangen, Germany
[6] Univ Piemonte Orientale, Dept Clin & Expt Med, Novara, Italy
[7] Vanderbilt Univ, Sch Med, Dept Med, Div Cardiovasc Med, Nashville, TN 37212 USA
关键词
rheumatic autoimmune disorders; guanylate binding protein-1; endothelial progenitor cells; inflammation; systemic lupus erythematosus; rheumatoid arthritis; systemic sclerosis; ENDOTHELIAL PROGENITOR CELLS; SYSTEMIC-LUPUS-ERYTHEMATOSUS; RHEUMATOID-ARTHRITIS; AUTOIMMUNE-DISEASES; PERIPHERAL-BLOOD; REVISED CRITERIA; ANGIOGENESIS; INTERFERON; GTPASE; CLASSIFICATION;
D O I
10.1111/j.1582-4934.2010.01146.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Rheumatic autoimmune disorders are characterized by a sustained pro-inflammatory microenvironment associated with impaired function of endothelial progenitor cells (EPC) and concomitant vascular defects. Guanylate binding protein-1 (GBP-1) is a marker and intracellular regulator of the inhibition of proliferation, migration and invasion of endothelial cells induced by several pro-inflammatory cytokines. In addition, GBP-1 is actively secreted by endothelial cells. In this study, significantly increased levels of GBP-1 were detected in the sera of patients with chronic inflammatory disorders. Accordingly we investigated the function of GBP-1 in EPC. Interestingly, stable expression of GBP-1 in T17b EPC induced premature differentiation of these cells, as indicated by a robust up-regulation of both Flk-1 and von Willebrand factor expression. In addition, GBP-1 inhibited the proliferation and migration of EPC in vitro. We confirmed that GBP-1 inhibited vessel-directed migration of EPC at the tissue level using the rat arterio-venous loop model as a novel quantitative in vivo migration assay. Overall, our findings indicate that GBP-1 contributes to vascular dysfunction in chronic inflammatory diseases by inhibiting EPC angiogenic activity via the induction of premature EPC differentiation.
引用
收藏
页码:1582 / 1592
页数:11
相关论文
共 46 条
[1]   Effect of anti-TNFα treatment on circulating endothelial progenitor cells (EPCs) in rheumatoid arthritis [J].
Ablin, Jacob N. ;
Boguslavski, Viktoria ;
Aloush, Valerie ;
Elkayam, Ori ;
Paran, Daphna ;
Caspi, Dan ;
George, Jacob .
LIFE SCIENCES, 2006, 79 (25) :2364-2369
[2]   Evaluation of blood vessel ingrowth in fibrin gel subject to type and concentration of growth factors [J].
Arkudas, A. ;
Tjiawi, J. ;
Saumweber, A. ;
Beier, J. P. ;
Polykandriotis, E. ;
Bleiziffer, O. ;
Horch, R. E. ;
Kneser, U. .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2009, 13 (9A) :2864-2874
[3]   Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model [J].
Arkudas, Andreas ;
Tjiawi, Jimmy ;
Bleiziffer, Oliver ;
Grabinger, Lucia ;
Polykandriotis, Elias ;
Beier, Justus P. ;
Stuerzl, Michael ;
Horch, Raymund E. ;
Kneser, Ulrich .
MOLECULAR MEDICINE, 2007, 13 (9-10) :480-487
[4]   THE AMERICAN-RHEUMATISM-ASSOCIATION 1987 REVISED CRITERIA FOR THE CLASSIFICATION OF RHEUMATOID-ARTHRITIS [J].
ARNETT, FC ;
EDWORTHY, SM ;
BLOCH, DA ;
MCSHANE, DJ ;
FRIES, JF ;
COOPER, NS ;
HEALEY, LA ;
KAPLAN, SR ;
LIANG, MH ;
LUTHRA, HS ;
MEDSGER, TA ;
MITCHELL, DM ;
NEUSTADT, DH ;
PINALS, RS ;
SCHALLER, JG ;
SHARP, JT ;
WILDER, RL ;
HUNDER, GG .
ARTHRITIS AND RHEUMATISM, 1988, 31 (03) :315-324
[5]   Isolation of putative progenitor endothelial cells for angiogenesis [J].
Asahara, T ;
Murohara, T ;
Sullivan, A ;
Silver, M ;
vanderZee, R ;
Li, T ;
Witzenbichler, B ;
Schatteman, G ;
Isner, JM .
SCIENCE, 1997, 275 (5302) :964-967
[6]   VEGF contributes to postnatal neovascularization by mobilizing bone marrow-derived endothelial progenitor cells [J].
Asahara, T ;
Takahashi, T ;
Masuda, H ;
Kalka, C ;
Chen, DH ;
Iwaguro, H ;
Inai, Y ;
Silver, M ;
Isner, JM .
EMBO JOURNAL, 1999, 18 (14) :3964-3972
[7]   Endothelial progenitor cells and rheumatic disorders [J].
Avouac, Jerome ;
Uzan, Georges ;
Kahan, Andre ;
Boileau, Catherine ;
Allanore, Yannick .
JOINT BONE SPINE, 2008, 75 (02) :131-137
[8]   Type I interferon in systemic lupus erythematosus and other autoimmune diseases [J].
Banchereau, Jacques ;
Pascual, Virginia .
IMMUNITY, 2006, 25 (03) :383-392
[9]   UP-REGULATION OF ENDOTHELIAL-CELL ADHESION MOLECULES CHARACTERIZES DISEASE-ACTIVITY IN SYSTEMIC LUPUS-ERYTHEMATOSUS - THE SHWARTZMAN PHENOMENON REVISITED [J].
BELMONT, HM ;
BUYON, J ;
GIORNO, R ;
ABRAMSON, S .
ARTHRITIS AND RHEUMATISM, 1994, 37 (03) :376-383
[10]   T17b murine embryonal endothelial progenitor cells can be induced towards both proliferation and differentiation in a fibrin matrix [J].
Bleiziffer, Oliver ;
Horch, Raymund E. ;
Hammon, Matthias ;
Arkudas, Andreas ;
Naschberger, Elisabeth ;
Rath, Subha ;
Pryymachuk, Galyna ;
Beier, Justus P. ;
Hatzopoulos, Antonis K. ;
Stuerzl, Michael ;
Kneser, Ulrich .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2009, 13 (05) :926-935