Production of recombinant porcine tumor necrosis factor alpha in a baculovirus expression system

被引：1

作者：

Ogikubo, Y

Mori, Y

Kojima, A

Tamura, Y

Yokomizo, Y

机构：

[1] Natl Vet Assay Lab, Tokyo 1858511, Japan

[2] Natl Inst Anim Hlth, Ibaraki 3050856, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1998年 / 60卷 / 11期

关键词：

baculovirus; porcine; tumor necrosis factor-alpha;

D O I：

10.1292/jvms.60.1203

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Porcine tumor necrosis factor alpha (TNF-alpha) was produced using a baculovirus system in Spodoptera frugiperda (SF21AE) cells. Cytotoxic activity was detected in supernatant of sonicated SF21AE cells infected with recombinant viruses. The recombinant protein was also demonstrated to be functionally active by its ability to cause apoptosis in Wehi 164 cells. Three distinct bands of 26, 17 and 14 KDa were revealed by Western blot analysis using anti-human TNF-alpha antibody. Moreover, the anti-human TNF-alpha antiserum significantly neutralized the cytotoxic activity of the supernatant of sonicated SF21AE cells infected with recombinant viruses.

引用

页码：1203 / 1207

页数：5

共 20 条

[1] IMPROVED BIOASSAY FOR THE DETECTION OF PORCINE TUMOR-NECROSIS-FACTOR USING A HOMOLOGOUS CELL-LINE - PK(15) [J].