共 45 条
Unsuspected pathway of the allosteric transition in hemoglobin
被引:67
作者:
Fischer, Stefan
[1
]
Olsen, Kenneth W.
[2
]
Nam, Kwangho
[3
]
Karplus, Martin
[3
,4
]
机构:
[1] Heidelberg Univ, Interdisziplinares Zentrum Wissenschaftliches Rec, D-69120 Heidelberg, Germany
[2] Loyola Univ, Dept Chem, Chicago, IL 60626 USA
[3] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
[4] Univ Strasbourg, Inst Sci & Ingn Supramol, Lab Chim Biophys, F-67000 Strasbourg, France
来源:
基金:
美国国家卫生研究院;
关键词:
conformational change;
cooperativity;
domain motion;
protein hinges;
QUATERNARY STRUCTURE;
MOLECULAR-DYNAMICS;
LIGAND-BINDING;
MECHANISM;
MOTIONS;
ENERGY;
MODEL;
MINIMIZATION;
ACTIVATION;
SIMULATION;
D O I:
10.1073/pnas.1011995108
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Large conformational transitions play an essential role in the function of many proteins, but experiments do not provide the atomic details of the path followed in going from one end structure to the other. For the hemoglobin tetramer, the transition path between the unliganded (T) and tetraoxygenated (R) structures is not known, which limits our understanding of the cooperative mechanism in this classic allosteric system, where both tertiary and quaternary changes are involved. The conjugate peak refinement algorithm is used to compute an unbiased minimum energy path at atomic detail between the two end states. Although the results confirm some of the proposals of Perutz [Perutz MF (1970) Stereochemistry of cooperative effects in haemoglobin. Nature 228: 726-734], the subunit motions do not follow the textbook description of a simple rotation of one alpha beta-dimer relative to the other. Instead, the path consists of two sequential quaternary rotations, each involving different subdomains and axes. The quaternary transitions are preceded and followed by phases of tertiary structural changes. The results explain the recent photodissociation measurements, which suggest that the quaternary transition has a fast (2 mu s) as well as a slow (20 mu s) component and provide a testable model for single molecule FRET experiments.
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页码:5608 / 5613
页数:6
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