Orchestrating the Biosynthesis of an Unnatural Pyrrolysine Amino Acid for Its Direct Incorporation into Proteins Inside Living Cells

被引：32

作者：

Ehrlich, Michael ^{[1
]}

Gattner, Michael J. ^{[1
]}

Viverge, Bastien ^{[1
]}

Bretzler, Johanna ^{[1
]}

Eisen, David ^{[1
]}

Stadlmeier, Michael ^{[1
]}

Vrabel, Milan ^{[2
]}

Carell, Thomas ^{[1
]}

机构：

[1] Univ Munich, Dept Chem, Ctr Integrat Prot Sci, D-81377 Munich, Germany

[2] Acad Sci Czech Republic, Inst Organ Chem & Biochem, CR-16610 Prague, Czech Republic

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2015年 / 21卷 / 21期

关键词：

amber suppression; bioorganic chemistry; pyrrolysine; synthetic biology; unnatural amino acid; PYRROLINE-CARBOXY-LYSINE; CLICK CHEMISTRY; GENETIC-CODE; TRANSFER-RNA; REACTION-MECHANISM; INSIGHTS; UAG;

D O I：

10.1002/chem.201500971

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We here report the construction of an E. coli expression system able to manufacture an unnatural amino acid by artificial biosynthesis. This can be orchestrated with incorporation into protein by amber stop codon suppression inside a living cell. In our case an alkyne-bearing pyrrolysine amino acid was biosynthesized and incorporated site-specifically allowing orthogonal double protein labeling.

引用

页码：7701 / 7704

页数：4

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