Peroxisome proliferator-activated receptor-coactivator 1-beta (PGC-1β) modulates the expression of genes involved in adipogenesis during preadipocyte differentiation in chicken

被引：7
作者：
Song, Weizhen [1 ]
Zhong, Chenglin [1 ]
Yuan, Yuncong [1 ]
Zhu, Qing [1 ]
Wang, Yan [1 ]
Yin, Huadong [1 ]
Li, Diyan [1 ]
Zhang, Zhichao [1 ]
Shu, Gang [2 ]
Yang, Chaowu [3 ]
Du, Huarui [3 ]
Jiang, Xiaosong [3 ]
Zhao, Xiaoling [1 ]
机构：
[1] Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key, Apt 211, Chengdu, Sichuan, Peoples R China
[2] Sichuan Agr Univ, Coll Vet Med, Dept Pharm, Chengdu, Sichuan, Peoples R China
[3] Sichuan Anim Sci Acad, Anim Breeding & Genet Key Lab Sichuan Prov, Chengdu 610066, Peoples R China
来源：
GENE | 2020年 / 741卷
基金：
中国国家自然科学基金;
关键词：
Adipocyte; Lipid accumulation; Differentiation; Peroxisome proliferator-activated receptor-coactivator 1-beta; RNA interference; MITOCHONDRIAL BIOGENESIS; PGC-1-ALPHA;
D O I：
10.1016/j.gene.2020.144516
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
To study the influence of the PGC-1 beta gene on chicken adipocyte proliferation and differentiation, we constructed RNA interference (RNAi) vectors that target the PGC-1 beta gene and transfected these vectors into adipocytes. Oil Red O staining and a CCK-8 cell kit were used to determine cell triglyceride accumulation status and cell proliferation after transfection, respectively. The mRNA abundances of PGC-1 beta and adipocyte-differentiation-related genes (PPAR., C/EBPa, SREBP-1c, FAS, and A-FABP) were detected by real-time PCR. The results showed that the mRNA and protein abundances of PGC-1 beta in PGC-1 beta-shRNA transfected adipocytes were significantly lower than those in the control. Interference decreased cell differentiation, but did not depress the cell proliferation. PGC-1 beta interference impeded the triglyceride accumulation, the mRNA expression levels of nuclear receptors PPAR. and SREBP-1c, and fatty acid synthetase (FAS), and both proteins PPAR. and SREBP-1c, and the fatty acids transporting protein A-FABP. Generally, PGC-1 beta modulated the cell differentiation and triglyceride accumulation in chicken adipocytes.
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页数：8
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