Cross-protective Immunity Against Leptospirosis Elicited by a Live, Attenuated Lipopolysaccharide Mutant

被引：54
作者：
Srikram, Amporn [3 ,5 ]
Zhang, Kunkun [1 ,6 ]
Bartpho, Thanatchaporn [3 ]
Lo, Miranda [1 ]
Hoke, David E. [2 ]
Sermswan, Rasana W. [4 ]
Adler, Ben [1 ,6 ]
Murray, Gerald L. [1 ]
机构：
[1] Monash Univ, Dept Microbiol, Clayton, Vic 3800, Australia
[2] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia
[3] Khon Kaen Univ, Fac Med, Melioidosis Res Ctr, Khon Kaen, Thailand
[4] Khon Kaen Univ, Fac Med, Dept Biochem, Khon Kaen, Thailand
[5] Kasetsart Univ, Fac Nat Resources & Agroind, Sect Food Technol, Sakon Nakhon, Thailand
[6] Monash Univ, Australian Res Council Ctr Excellence Struct & Fu, Clayton, Vic 3800, Australia
来源：
JOURNAL OF INFECTIOUS DISEASES | 2011年 / 203卷 / 06期
基金：
英国医学研究理事会; 澳大利亚研究理事会;
关键词：
OUTER-MEMBRANE PROTEINS; PATHOGENIC LEPTOSPIRA; LETHAL INFECTION; INTERROGANS REQUIRES; HEME OXYGENASE; HAMSTERS; VACCINE; EXPRESSION; POMONA; LIPL32;
D O I：
10.1093/infdis/jiq127
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Methods. We evaluated an attenuated transposon mutant of Leptospira interrogans serovar Manilae (M1352, defective in lipopolysaccharide biosynthesis) as a live vaccine against leptospirosis. Hamsters received a single dose of vaccine and were challenged with the homologous serovar (Manilae) and a serologically unrelated heterologous serovar (Pomona). Comparisons were made with killed vaccines. Potential cross-protective antigens against leptospirosis were investigated. Results. Live M1352 vaccine induced superior protection in hamsters against homologous challenge. The live vaccine also stimulated cross-protection against heterologous challenge, with 100% survival (live M1352) versus 40% survival (killed vaccine). Hamsters receiving either vaccine responded to the dominant membrane proteins LipL32 and LipL41. Hamsters receiving the live vaccine additionally recognized LA3961/OmpL36 (unknown function), Loa22 (OmpA family protein, recognized virulence factor), LA2372 (general secretory protein G), and LA1939 (hypothetical protein). Manilae LigA was recognized by M1352 vaccinates, whereas LipL36 was detected in Pomona. Conclusion. This study demonstrated that a live, attenuated vaccine can stimulate cross-protective immunity to L. interrogans and has identified antigens that potentially confer cross-protection against leptospirosis.
引用
收藏
页码：870 / 879
页数：10
相关论文
共 46 条
[1]   IMMUNOGENICITY OF BOILED COMPARED WITH FORMALIZED LEPTOSPIRAL VACCINES IN RABBITS, HAMSTERS AND HUMANS [J].
ADLER, B ;
FAINE, S .
JOURNAL OF HYGIENE, 1980, 84 (01) :1-10
[2]   SUSCEPTIBILITY OF MICE TREATED WITH CYCLOPHOSPHAMIDE TO LETHAL INFECTION WITH LEPTOSPIRA-INTERROGANS SEROVAR POMONA [J].
ADLER, B ;
FAINE, S .
INFECTION AND IMMUNITY, 1976, 14 (03) :703-708
[3]   HOST IMMUNOLOGICAL MECHANISMS IN RESISTANCE OF MICE TO LEPTOSPIRAL INFECTIONS [J].
ADLER, B ;
FAINE, S .
INFECTION AND IMMUNITY, 1977, 17 (01) :67-72
[4]   ANTIBODIES INVOLVED IN HUMAN IMMUNE-RESPONSE TO LEPTOSPIRAL INFECTION [J].
ADLER, B ;
FAINE, S .
JOURNAL OF MEDICAL MICROBIOLOGY, 1978, 11 (04) :387-400
[5]   Leprospira and leptospirosis [J].
Adler, Ben ;
de la Pena Moctezuma, Alejandro .
VETERINARY MICROBIOLOGY, 2010, 140 (3-4) :287-296
[6]  
BABUDIER.B, 1973, B WORLD HEALTH ORGAN, V48, P587
[7]   Expression and distribution of leptospiral outer membrane components during renal infection of hamsters [J].
Barnett, JK ;
Barnett, D ;
Bolin, CA ;
Summers, TA ;
Wagar, EA ;
Cheville, NF ;
Hartskeerl, RA ;
Haake, DA .
INFECTION AND IMMUNITY, 1999, 67 (02) :853-861
[8]   Use of a monovalent leptospiral vaccine to prevent venal colonization and urinary shedding in cattle exposed to Leptospira borgpetersenii serovar hardjo [J].
Bolin, CA ;
Alt, DP .
AMERICAN JOURNAL OF VETERINARY RESEARCH, 2001, 62 (07) :995-1000
[9]   Random Insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon [J].
Bourhy, P ;
Louvel, H ;
Saint Girons, I ;
Picardeau, M .
JOURNAL OF BACTERIOLOGY, 2005, 187 (09) :3255-3258
[10]   Genome reduction in Leptospira borgpetersenii reflects limited transmission potential [J].
Bulach, Dieter M. ;
Zuerner, Richard L. ;
Wilson, Peter ;
Seemann, Torsten ;
McGrath, Annette ;
Cullen, Paul A. ;
Davis, John ;
Johnson, Matthew ;
Kuczek, Elizabeth ;
Alt, David P. ;
Peterson-Burch, Brooke ;
Coppel, Ross L. ;
Rood, Julian I. ;
Davies, John K. ;
Adler, Ben .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (39) :14560-14565
12345