Rapid Identification of Bioactive Compounds Reducing the Production of Amyloid β-Peptide (Aβ) from South African Plants Using an Automated HPLC/SPE/HPLC Coupling System

被引：9

作者：

Kwon, Hak Cheol ^{[2
]}

Cha, Jin Wook ^{[2
]}

Park, Jin-Soo ^{[2
]}

Chun, Yoon Sun ^{[1
]}

Moodley, Nivan ^{[3
]}

Maharaj, Vinesh J. ^{[3
]}

Youn, Sung Hee ^{[1
]}

Chung, Sungkwon ^{[1
]}

Yang, Hyun Ok ^{[2
]}

机构：

[1] Sungkyunkwan Univ, Sch Med, Dept Physiol, Samsung Biomed Res Inst, Suwon 440746, South Korea

[2] Korea Inst Sci & Technol, Nat Prod Res Ctr, Kangnung 210340, South Korea

[3] RSA, CSIR, ZA-0001 Pretoria, South Africa

来源：

BIOMOLECULES & THERAPEUTICS | 2011年 / 19卷 / 01期

关键词：

HPLC/SPE/HPLC; Alzheimer's disease; Amyloid beta-peptide; Euclea crispa subsp crispa; Crinum macowanii; FAMILIAL ALZHEIMERS-DISEASE; NATURAL-PRODUCTS; EUCLEA-NATALENSIS; CRINUM-MACOWANII; DRUG DISCOVERY; SENILE PLAQUES; ALKALOIDS; AMARYLLIDACEAE; TRITERPENOIDS; CONSTITUENTS;

D O I：

10.4062/biomolther.2011.19.1.090

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Automated HPLC/SPE/HPLC coupling experiments using the Sepbox system allowed the rapid identification of four bioactive principles reducing the production of amyloid beta-peptide (A beta) from two South African plants, Euclea crispa subsp. crispa and Crinum macowanii. The structures of biologically active compounds isolated from the methanol extract of Euclea crispa subsp. crispa were assigned as 3-oxo-oleanolic acid (1) and natalenone (2) based on their NMR and MS data, while lycorine (3) and hamayne (4) were isolated from the dichloromethane-methanol (1:1) extract of Crinum macowanii. These compounds were shown to inhibit the production of A beta from He La cells stably expressing Swedish mutant form of amyloid precursor protein (APPsw).

引用

页码：90 / 96

页数：7

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