A New Method in CNS (Central Nervous System) In Vitro Cultures in the Mouse: Study of Effectiveness

被引:0
作者
Erdogan, Ender [1 ]
Ozturk, Gurkan [2 ,3 ]
Ragbetli, Murat Cetin [4 ]
机构
[1] Selcuk Univ, Selcuklu Tip Fak, Histol & Embriyol Anabilim Dali, TR-42075 Konya, Turkey
[2] Yuzuncu Yil Univ, Tip Fak, Fizyol Anabilim Dali, TR-65200 Van, Turkey
[3] Yuzuncu Yil Univ, Tip Fak, Neurosci Arastirma Birimi, TR-65200 Van, Turkey
[4] Yuzuncu Yil Univ, Tip Fak, Histol & Embriyol Anabilim Dali, TR-65200 Van, Turkey
关键词
Mice; Central nervous system; Culture; Slice section; Confocal microscope; ORGANOTYPIC SLICE CULTURES; BRAIN-SLICES; CELL-DEATH; HIPPOCAMPAL; TISSUE; ASSAY; PROLIFERATION; CYTOTOXICITY; FLUORESCENCE; VIABILITY;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In this study: to evaluate the effectiveness of collagen coating method, using in the peripheral nervous system cultures. and its involving factors caused from manipulations in central nervous system (CNS) cultures was aimed. Via frontal approach, brains, transected from young Swiss albino mice, were taken into artificial cerebro-spinal fluid immediately and made blocks in agarose gel. With a vibration microtome, 200 pm thickness horizontally live slices were taken in to the dishes filled with culture medium. Tissue sections were analyzed as two groups. In the group 1 (control): fresh slices were evaluated directly. In the group 2: sections were covered with collagen gel (Type I) and left in the incubator (5% CO(2)) for 3 days. These sections were dyed with calcein and propidium iodide for viability and non-viability and then observed with confocal laser scanning microscope. Images were captured digitally and examined. Since negative effects of high melting temperature of standard agar on the livability, using low melting agar to tissue blocking and high frequency - low speed vibrotome setting to cut were more preferably. In the 3 days cultures, viability/nonviability rates were indicated better values. It is concluded that, in the CNS slicing cultures, collagen coating method was an easier, effective, useful and alternative method to present techniques.
引用
收藏
页码:1037 / 1043
页数:7
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