Non-uniform flow behavior in a parallel plate flow chamber alters endothelial cell responses

被引:42
作者
McCann, JA
Peterson, SD
Plesniak, MW
Webster, TJ
Haberstroh, KM
机构
[1] Purdue Univ, Weldon Sch Biomed Engn, Dept Biomed Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Sch Mech Engn, Maurice J Zucrow Labs, W Lafayette, IN 47907 USA
关键词
micro-PIV; laminar flow; flow variations; mRNA expression; vascular cells;
D O I
10.1007/s10439-005-1735-9
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Arterial flow characteristics determine vessel health by modulating vascular endothelial cells. One system used to study these interactions is the parallel plate flow chamber. The present in vitro study quantified the uniformity of fluid flow across a parallel plate flow chamber and characterized plate-location dependent endothelial cell gene expression. More specifically, shear stress varied by as much as 11% across the chamber area, which caused non-uniform ecNOS (p < 0.05) and COX-2 (p < 0.05) mRNA expression across the plate area. Results herein suggest that chamber variations may result during construction or assembly, which ultimately affect flow-sensitive cell responses (including mRNA expression). Therefore, these limitations should be considered when reporting endothelial cell responses to fluid flow using parallel plate flow chambers.
引用
收藏
页码:328 / 336
页数:9
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