On our way to sub-second separations of enantiomers in high-performance liquid chromatography

被引：32
作者：
Khundadze, Nana [1 ]
Pantsulaia, Salome [1 ]
Fanali, Chiara [2 ]
Farkas, Tivadar [3 ]
Chankvetadze, Bezhan [1 ]
机构：
[1] Tbilisi State Univ, Sch Exact & Nat Sci, Inst Phys & Analyt Chem, Chavchavadze Ave 3, GE-0179 Tbilisi, Georgia
[2] Univ Campus Biomed Rome, Dept Med, Via Alvaro del Portillo 21, I-00128 Rome, Italy
[3] Phenomenex Inc, 411 Madrid Ave, Torrance, CA 90501 USA
来源：
JOURNAL OF CHROMATOGRAPHY A | 2018年 / 1572卷
关键词：
Enantioseparations; Chiral stationary phases; Segmented chiral-achiral columns; Fast separation of enantiomers; SUPERFICIALLY POROUS PARTICLES; CHIRAL STATIONARY PHASES; CAPILLARY COLUMNS; SILICA PARTICLES; HIGH-EFFICIENCY; CELLULOSE; ENANTIOSEPARATIONS; SPEED;
D O I：
10.1016/j.chroma.2018.08.027
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study our preliminary attempt for obtaining fast and highly efficient separations of enantiomers in high-performance liquid chromatography with slightly modified state-of-the-art commercial instrumentation is described. In order to reach this goal after careful selection of chiral analytes, the preparation of chiral stationary phase (CSP), mobile phase composition and column dimensions were optimized. The concept of segmented chiral-achiral column was introduced. As the result of these optimizations baseline separation of enantiomers was achieved with the analysis time between 1-2 s. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：37 / 43
页数：7
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