NRSF plays a regulatory role in human insulin gene transcription

被引：0

作者：

Liu Qing-Bin ^{[1
]}

Li Yan-Hua ^{[1
]}

Yang Yin-Xiang ^{[1
]}

Yuan Hong-Feng ^{[1
]}

Zhang Rui ^{[1
]}

Qin Li-Peng ^{[1
]}

Lu Yang ^{[1
]}

Zhang Sai-Nan ^{[1
]}

Sun Di-Jin ^{[1
]}

Chen Lin ^{[1
]}

Bai Ci-Xian ^{[1
]}

Nan Xue ^{[1
]}

Wang Yun-Fang ^{[1
]}

Pei Xue-Tao ^{[1
]}

机构：

[1] Beijing Inst Transfus Med, Lab Stem Cell Biol & Regenerat Med, Beijing 100850, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2007年 / 34卷 / 09期

关键词：

neuronal restrictive silencer factor (NRSF); neuronal restrictive silencer element (NRSE); insulin; promoter;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcriptional repressor REI silencer transcription factor (NRSF/REST) is an important factor that restricts some neuronal traits in neurons. Since these traits are also present in pancreatic islet cells, NRSF-regulated genes involved in islet function are searched. A NRSE-like motif was analysed in human insulin promoter. The role of NRSE was evaluated by generating a model of insulin-secreting cells that firmly express NRSF. The presence of NRSF led to a decrease in activity of human insulin promoter by stable or transient transfection with human insulin-promoter luciferase. The predicted NRSE-like motif also confers NRSF-dependent transcriptional repression in the context of a surrogate gene promoter. Specific binding activity of NRSF/REST to the NRSE-like motif was confirmed by EMSA. Moreover, the binding activity is competed by consensus NRSE sequence. These data showed that human insulin promoter is regulated by the transcriptional repressor NRSF/REST via the NRSE-like motif.

引用

页码：952 / 959

页数：8

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