Lipidomic Analysis to Assess Oxidative Stress in Acute Coronary Syndrome and Acute Stroke Patients

被引:13
作者
Maly, Martin [1 ,2 ]
Hajsl, Martin [1 ,2 ]
Bechynska, Kamila [3 ]
Kucerka, Ondrej [1 ,2 ]
Sramek, Martin [2 ,4 ]
Suttnar, Jiri [5 ]
Hlavackova, Alzbeta [5 ]
Hajslova, Jana [3 ]
Kosek, Vit [3 ]
机构
[1] Charles Univ Prague, Fac Med 1, Dept Med, Vojenske Nemocnice 1200, Prague 16900, Czech Republic
[2] Mil Univ Hosp, Vojenske Nemocnice 1200, Prague 16900, Czech Republic
[3] Univ Chem & Technol, Dept Food Chem & Anal, Tech 3, Prague 16628, Czech Republic
[4] Charles Univ Prague, Fac Med 1, Comprehens Stroke Ctr, Vojenske Nemocnice 1200, Prague 16900, Czech Republic
[5] Inst Hematol & Blood Transfus, Nemocnice 2094, Prague 12820, Czech Republic
关键词
acute coronary syndrome; stroke; plasma; lipidomics; high-resolution mass spectrometry; LIPOPROTEIN-LIPASE; HUMAN-PLASMA; IDENTIFICATION; METABOLOMICS; BIOMARKERS;
D O I
10.3390/metabo11070412
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alterations in lipid metabolism mediated by oxidative stress play a key role in the process of atherosclerosis and superimposed thrombosis; these can lead to acute coronary syndrome (ACS) and acute ischemic stroke (AIS). Multiple studies have shown that the formation of atheromatous lesions is initiated by oxidation of low-density lipoproteins incorporated into the intima of the vessel wall. Here, we studied lipids in plasma samples from three cohorts: 61 patients with ACS (group A), 49 patients with AIS (group D), and 82 controls (group K). Untargeted lipidomics based on high-performance liquid chromatography coupled to mass spectrometry (UHPLC-HRMS) was employed to obtain comprehensive information on whether relationships exist between these patient categories based on lipid patterns. In addition, malondialdehyde (MDA) as a standard marker of oxidative stress was monitored. The most characteristic lipids in group K were fatty acyls of hydroxyfatty acids (FAHFAs). As expected, MDA concentrations were the lowest in group K. Our findings can better explain ongoing pathologies, both acute and chronic, with the potential for future diagnosis and treatment.
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页数:14
相关论文
共 26 条
  • [1] A Comparative Metabolomics Approach Reveals Early Biomarkers for Metabolic Response to Acute Myocardial Infarction
    Ali, Sara E.
    Farag, Mohamed A.
    Holvoet, Paul
    Hanafi, Rasha S.
    Gad, Mohamed Z.
    [J]. SCIENTIFIC REPORTS, 2016, 6
  • [2] Systematic review on the risk and benefit of different cholesterol-lowering interventions
    Bucher, HC
    Griffith, LE
    Guyatt, GH
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1999, 19 (02) : 187 - 195
  • [3] A Review of Lipidomics of Cardiovascular Disease Highlights the Importance of Isolating Lipoproteins
    Ding, Ming
    Rexrode, Kathryn M.
    [J]. METABOLITES, 2020, 10 (04)
  • [4] A comprehensive classification system for lipids
    Fahy, E
    Subramaniam, S
    Brown, HA
    Glass, CK
    Merrill, AH
    Murphy, RC
    Raetz, CRH
    Russell, DW
    Seyama, Y
    Shaw, W
    Shimizu, T
    Spener, F
    van Meer, G
    VanNieuwenhze, MS
    White, SH
    Witztum, JL
    Dennis, EA
    [J]. JOURNAL OF LIPID RESEARCH, 2005, 46 (05) : 839 - 861
  • [5] Tryptophan Metabolism, Inflammation, and Oxidative Stress in Patients with Neurovascular Disease
    Hajsl, Martin
    Hlavackova, Alzbeta
    Broulikova, Karolina
    Sramek, Martin
    Maly, Martin
    Dyr, Jan E.
    Suttnar, Jiri
    [J]. METABOLITES, 2020, 10 (05)
  • [6] LIPOPROTEIN LIPASE-CATALYZED HYDROLYSIS OF PHOSPHOLIPID MONOLAYERS - EFFECT OF FATTY ACYL COMPOSITION ON ENZYME-ACTIVITY
    JACKSON, RL
    DEMEL, RA
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 128 (02) : 670 - 675
  • [7] LipidMatch: an automated workflow for rule-based lipid identification using untargeted high-resolution tandem mass spectrometry data
    Koelmel, Jeremy P.
    Kroeger, Nicholas M.
    Ulmer, Candice Z.
    Bowden, John A.
    Patterson, Rainey E.
    Cochran, Jason A.
    Beecher, Christopher W. W.
    Garrett, Timothy J.
    Yost, Richard A.
    [J]. BMC BIOINFORMATICS, 2017, 18
  • [8] KOSTNER GM, 1983, ADV LIPID RES, V20, P1
  • [9] Plasma metabolomics reveals a potential panel of biomarkers for early diagnosis in acute coronary syndrome
    Laborde, Carlos M.
    Mourino-Alvarez, Laura
    Posada-Ayala, Maria
    Alvarez-Llamas, Gloria
    Gomez Serranillos-Reus, Manuel
    Moreu, Jose
    Vivanco, Fernando
    Padial, Luis R.
    Barderas, Maria G.
    [J]. METABOLOMICS, 2014, 10 (03) : 414 - 424
  • [10] Metabolomics through the lens of precision cardiovascular medicine
    Lam, Sin Man
    Wang, Yuan
    Li, Bowen
    Du, Jie
    Shui, Guanghou
    [J]. JOURNAL OF GENETICS AND GENOMICS, 2017, 44 (03) : 127 - 138