Plasma kallikrein contributes to ambient particulate matter-induced lung injury

被引:10
|
作者
Wang, Bo [1 ]
Yan, Xiaofeng [1 ]
Chen, Fengwu [1 ]
Yang, Aizhen [1 ]
Lu, Yi [2 ]
Wu, Yi [1 ,3 ]
机构
[1] Soochow Univ, Cyrus Tang Hematol Ctr, Collaborat Innovat Ctr Hematol, State Key Lab Radiat Med & Protect, Suzhou, Peoples R China
[2] Wuhan Thalys Med Technol Inc, Wuhan, Peoples R China
[3] Temple Univ, Sol Sherry Thrombosis Res Ctr, Sch Med, 3420 North Broad St, Philadelphia, PA 19140 USA
关键词
Plasma kallikrein; Particulate matter 2.5; Lung injury; Coagulation; Inflammation; MOLECULAR-WEIGHT KININOGEN; AIR-POLLUTION; INFLAMMATION; FINE; ACTIVATION; EXPOSURE; PREKALLIKREIN; BRADYKININ; COMPLEX; CELLS;
D O I
10.1016/j.bbrc.2019.07.060
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Particulate matter (PM) is a key component of air pollutants and is associated with mortality of cardiovascular and respiratory diseases. PM-induced tissue injury involves inflammation and coagulation. Plasma prekallikrein (pKal), along with coagulation factor XII (FXII) and high-molecular-weight kininogen (HK), form the plasma kallikrein-kinin system (KKS), a component of the innate immune response that generates proinflammatory products in response to injury. When the KKS proteins contact with activation surface such as negatively charged molecules, this system becomes activated. Activated kallikrein (Kal) activates FXII to initiate the intrinsic coagulation pathway, and cleaves HK to release bradykinin to enhance vascular permeability and systemic inflammation. In his study we determined the role of plasma pKal in the PM2.5-induced lung injury. Using TALEN technology, we generated a new mouse strain lacking the gene for pKal. In PM2.5-induced lung injury model, Klkb1(-/-) mice exhibited a decrease in total protein, cells numbers in bronchoalveolar lavage fluid (BALF) and histologic lung injury score. The TNIF-alpha and IL-6 levels in BALF were significantly decreased in PM2.5-treated Klkb1(-/-) mice. Plasma thrombin-antithrombin (TAT) complex levels were significantly decreased in PM2.5-treated Klkb1(-/-) mice. PM2.5 induces pKal activation, HK cleavage and bradykinin production. PM2.5-induced HK cleavage in plasma was completely blocked by a Kal inhibitor, as well as in pKal-deficient plasma. PM2.5 markedly induced thrombin generation in human plasma and wild-type mouse plasma, which was inhibited by both blockade and deficiency of pKal. Taken together, plasma pKal is activated by PM2.5 and the activated Kal plays an important role in PM2.5-induced lung injury. (C) 2019 Elsevier Inc. All rights reserved.
引用
收藏
页码:409 / 415
页数:7
相关论文
共 50 条
  • [1] Role of plasma kallikrein-kinin system in the pathogenesis of ambient particulate matter-induced lung injury
    Wang, B.
    Dai, J.
    Colmam, R. W.
    Wu, Y.
    JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2016, 14 : 64 - 65
  • [2] Necroptosis contributes to airborne particulate matter-induced ocular surface injury
    Shi, Kexin
    Yin, Qichuan
    Tang, Xiajing
    Yu, Xiaoning
    Zheng, Sifan
    Shentu, Xingchao
    TOXICOLOGY, 2022, 470
  • [3] Necroptosis Contributes to Urban Particulate Matter-Induced Airway Epithelial Injury
    Xu, Feng
    Luo, Man
    He, Lulu
    Cao, Yuan
    Li, Wen
    Ying, Songmin
    Chen, Zhihua
    Shen, Huahao
    CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018, 46 (02) : 699 - 712
  • [4] Therapeutic Effects of Cornuside on Particulate Matter-Induced Lung Injury
    Kim, Go Oun
    Park, Eui Kyun
    Park, Dong Ho
    Song, Gyu Yong
    Bae, Jong-Sup
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (05)
  • [5] Establishment of particulate matter-induced lung injury model in mouse
    Se Yong Park
    Kyu Sup An
    Buhyun Lee
    Ju-Hee Kang
    Hyun Jin Jung
    Min Woo Kim
    Hyeon Yeol Ryu
    Kyu-Suk Shim
    Ki Taek Nam
    Yeo Sung Yoon
    Seung Hyun Oh
    Laboratory Animal Research, 37
  • [6] Establishment of particulate matter-induced lung injury model in mouse
    Park, Se Yong
    An, Kyu Sup
    Lee, Buhyun
    Kang, Ju-Hee
    Jung, Hyun Jin
    Kim, Min Woo
    Ryu, Hyeon Yeol
    Shim, Kyu-Suk
    Nam, Ki Taek
    Yoon, Yeo Sung
    Oh, Seung Hyun
    LABORATORY ANIMAL RESEARCH, 2021, 37 (01)
  • [7] Sirt1 Protects Against Particulate Matter-Induced Lung Injury
    Fu, J.
    Wu, Z.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2015, 191
  • [8] Aerobic exercise ameliorates particulate matter-induced lung injury in aging rats
    Qin, Fei
    Cui, Shuqiang
    Dong, Yanan
    Xu, Minxiao
    Wang, Zhongwei
    Qu, Chaoyi
    Zhao, Jiexiu
    ENVIRONMENTAL POLLUTION, 2021, 280
  • [9] Particulate matter-induced epigenetic modifications and lung complications
    Afthab, Muhammed
    Hambo, Shadi
    Kim, Hyunji
    Alhamad, Ali
    Harb, Hani
    EUROPEAN RESPIRATORY REVIEW, 2024, 33 (174):
  • [10] Particulate matter-induced epigenetic changes and lung cancer
    Li, Jinghong
    Li, Willis X.
    Bai, Chunxue
    Song, Yuanlin
    CLINICAL RESPIRATORY JOURNAL, 2017, 11 (05): : 539 - 546