Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes

被引：147
作者：
Fu, Shulan [1 ,2 ]
Chen, Lei [1 ,2 ]
Wang, Yangyang [1 ,2 ]
Li, Meng [1 ,2 ]
Yang, Zujun [3 ]
Qiu, Ling [1 ,2 ]
Yan, Benju [1 ]
Ren, Zhenglong [1 ,2 ]
Tang, Zongxiang [1 ,2 ]
机构：
[1] Sichuan Agr Univ, Prov Key Lab Plant Breeding & Genet, Chengdu 611130, Sichuan, Peoples R China
[2] Sichuan Agr Univ, Agron Coll, Chengdu 611130, Sichuan, Peoples R China
[3] Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Sichuan, Peoples R China
来源：
SCIENTIFIC REPORTS | 2015年 / 5卷
基金：
中国国家自然科学基金;
关键词：
TRANSLOCATIONS; ORGANIZATION; RESISTANCE; CENTROMERE; SEQUENCES; CHROMATIN; LOCATION; 1R;
D O I：
10.1038/srep10552
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Genomic in situ hybridization (GISH) has been widely used to detect rye (Secale cereale L.) chromosomes in wheat (Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeling of genomic DNA of rye and denaturing of chromosomes and probes. In this study, new oligonucleotide probes Oligo-1162, Oligo-pSc200 and Oligo-pSc250 were developed. The three new probes can be used for non-denaturing fluorescence in situ hybridization (ND-FISH) assays and replace genomic DNA of rye as a probe to discriminate rye chromosomes in wheat backgrounds. In addition, previously developed oligonucleotide probes Oligo-pSc119.2-1, Oligo-pSc119.2-2, Oligo-pTa535-1, Oligo-pTa535-2, Oligo-pTa71-2, Oligo-pAWRC.1 and Oligo-CCS1 can also be used for ND-FISH of wheat and rye. These probes have provided an easier, faster and more cost-effective method for the FISH analysis of wheat and hybrids derived from wheat x rye.
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页数：7
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