Outside-in integrin signal transduction - alpha(IIb)beta(3)-(GP IIb-IIIa) tyrosine phosphorylation induced by platelet aggregation

alpha(IIb)beta(3)-(GPIIb-IIIa) is the most abundant integrin expressed on platelets and plays a critical role in platelet aggregation and normal hemostasis. In response to platelet stimulation by agonists such as thrombin, alpha(IIb)beta(3) becomes a receptor for the adhesive proteins fibrinogen, von Willebrand factor, vitronectin, and fibronectin. Binding of extracellular matrix ligands allows the integrin to transmit a signal to the inside of the cell, but the exact mechanisms whereby integrins transduce these signals remain unclear. In this paper we demonstrate that the beta(3) subunit of alpha(IIb)beta(3) was phosphorylated on tyrosine residues in response to thrombin induced platelet aggregation. However, tyrosine phosphorylation was not observed when platelets were stimulated by thrombin in the presence of an inhibitor of aggregation. Phosphotyrosine was only detected when platelets were solubilized under protein-denaturing conditions, A peptide corresponding to residues 740-762 of the beta(3) cytoplasmic domain was capable of binding the signaling proteins SHC and GRB2, GRB2 binding occurred only when both tyrosine residues (Tyr-747 and Tyr-759) were phosphorylated. SHC binding also occurred to a peptide monophosphorylated at Tyr-759, The data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside in signaling cascades by inducing association of signaling components directly with the integrin.