One-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-D-ribose

被引：12
作者：
Iwai, Noritaka [1 ]
Kitahara, Yuki [1 ]
Kitazume, Tomoya [1 ]
机构：
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268501, Japan
来源：
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2011年 / 73卷 / 1-4期
关键词：
Fluorine resource; Fluorinase; One-pot synthesis; 3-Step enzymatic reaction; S-ADENOSYLMETHIONINE SYNTHETASE; STREPTOMYCES-CATTLEYA; ESCHERICHIA-COLI; FLUORINATED MATERIALS; BOND FORMATION; IDENTIFICATION; BIOSYNTHESIS; FLUOROACETATE; MECHANISM; 4-FLUOROTHREONINE;
D O I：
10.1016/j.molcatb.2011.06.018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The one-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-D-ribose (5-FOR) from ATP and L-methionine using S-adenosyl-L-methionine synthase (MetK), fluorinase and methylthioadenosine nucleosidase (MtnN) in the presence of fluoride ion, was described. Especially, for the purpose of the sustainable development of fluorine chemistry, the reuse of fluoride ion generated from BF(4) ionic liquids and/or the biodegradation of BTF in the one-pot synthetic process to 5-FOR, was described. (C) 2011 Published by Elsevier B.V.
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页码：1 / 4
页数：4
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