Characterization of antioxidant polysaccharides from Auricularia auricular using microwave-assisted extraction

被引：261
作者：
Zeng, Wei-Cai [1 ,2 ]
Zhang, Zeng [3 ]
Gao, Hong [1 ]
Jia, Li-Rong [1 ]
Chen, Wu-Yong [2 ]
机构：
[1] Sichuan Univ, Coll Light Ind Text & Food Engn, Chengdu 610065, Peoples R China
[2] Sichuan Univ, Natl Engn Lab Clean Technol Leather Manufacture, Chengdu 610065, Peoples R China
[3] Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2012年 / 89卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Auricularia auricula; Polysaccharides; Microwave-assisted extraction; Characterization; Antioxidant activity; L; LEAF; FRACTIONS; BODIES;
D O I：
10.1016/j.carbpol.2012.03.078
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Influence of microwave-assisted extraction (MAE) to the characterization and corresponding antioxidant activity of polysaccharides from Auricularia auricula (AAP) was determined. According to gas chromatography-mass spectrometry, high performance size exclusion chromatography, Fourier transform infrared spectroscopy, partial acid hydrolysis, periodic acid oxidation, Smith degradation, methylation analysis and atomic force microscopy. AAP was a heteropolysaccharide (composed of glucose, galactose, mannose, arabinose and rhamnose at the molar ratio of 37.53:1:4.32:0.93:0.91) with the molecular weight of 2.77 x 10(4) Da, observed as a spherical lump, and the backbone of AAP was mainly composed by glucose with (1 -> 3) linked. Moreover. AAP exhibited significant in vitro antioxidant activity in scavenging free radicals (ABTS, DPPH, superoxide and hydroxyl radicals), in lipid peroxidation and reducing power assays. The present result suggested that AAP by MAE had low molecular weight and remarkable antioxidant capability. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：694 / 700
页数：7
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