Characterization of antioxidant polysaccharides from Auricularia auricular using microwave-assisted extraction

被引：261
作者：
Zeng, Wei-Cai [1 ,2 ]
Zhang, Zeng [3 ]
Gao, Hong [1 ]
Jia, Li-Rong [1 ]
Chen, Wu-Yong [2 ]
机构：
[1] Sichuan Univ, Coll Light Ind Text & Food Engn, Chengdu 610065, Peoples R China
[2] Sichuan Univ, Natl Engn Lab Clean Technol Leather Manufacture, Chengdu 610065, Peoples R China
[3] Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2012年 / 89卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Auricularia auricula; Polysaccharides; Microwave-assisted extraction; Characterization; Antioxidant activity; L; LEAF; FRACTIONS; BODIES;
D O I：
10.1016/j.carbpol.2012.03.078
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Influence of microwave-assisted extraction (MAE) to the characterization and corresponding antioxidant activity of polysaccharides from Auricularia auricula (AAP) was determined. According to gas chromatography-mass spectrometry, high performance size exclusion chromatography, Fourier transform infrared spectroscopy, partial acid hydrolysis, periodic acid oxidation, Smith degradation, methylation analysis and atomic force microscopy. AAP was a heteropolysaccharide (composed of glucose, galactose, mannose, arabinose and rhamnose at the molar ratio of 37.53:1:4.32:0.93:0.91) with the molecular weight of 2.77 x 10(4) Da, observed as a spherical lump, and the backbone of AAP was mainly composed by glucose with (1 -> 3) linked. Moreover. AAP exhibited significant in vitro antioxidant activity in scavenging free radicals (ABTS, DPPH, superoxide and hydroxyl radicals), in lipid peroxidation and reducing power assays. The present result suggested that AAP by MAE had low molecular weight and remarkable antioxidant capability. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：694 / 700
页数：7
相关论文
共 35 条
[1]   Microwave-assisted extraction of phenolic antioxidant compounds from peanut skins [J].
Ballard, Tameshia S. ;
Mallikarjunan, Parameswarakumar ;
Zhou, Kequan ;
O'Keefe, Sean .
FOOD CHEMISTRY, 2010, 120 (04) :1185-1192
[2]   Effect of polysaccharide from Auricularia auricula on blood lipid metabolism and lipoprotein lipase activity of ICR mice fed a cholesterol-enriched diet [J].
Chen, G. ;
Luo, Y. -C. ;
Ji, B. -P ;
Li, B. ;
Guo, Y. ;
Li, Y. ;
Su, W. ;
Xiao, Z. -L. .
JOURNAL OF FOOD SCIENCE, 2008, 73 (06) :H103-H108
[3]  
[陈钢 Chen Gang], 2010, [食品科学, Food Science], V31, P210
[4]   Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia Sinensis) [J].
Chen, Haixia ;
Zhang, Min ;
Qu, Zhishuang ;
Xie, Bijun .
FOOD CHEMISTRY, 2008, 106 (02) :559-563
[5]   Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum [J].
Chen, Yi ;
Xie, Ming-Yong ;
Nie, Shao-Ping ;
Li, Chang ;
Wang, Yuan-Xing .
FOOD CHEMISTRY, 2008, 107 (01) :231-241
[6]   Antioxidant activity of Piper betle L. leaf extract in vitro [J].
Dasgupta, N ;
De, B .
FOOD CHEMISTRY, 2004, 88 (02) :219-224
[7]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
[8]  
Eskilsson CS, 2000, J CHROMATOGR A, V902, P227
[9]   Evaluation of antioxidant property and quality of breads containing Auricularia auricula polysaccharide flour [J].
Fan, Lisheng ;
Zhang, Shenghua ;
Yu, Lin ;
Ma, Li .
FOOD CHEMISTRY, 2007, 101 (03) :1158-1163
[10]   Physico-chemical characterization of alkaline soluble polysaccharides from sugar beet pulp [J].
Fishman, Marshall L. ;
Chau, Hoa K. ;
Cooke, Peter H. ;
Yadav, Madhav P. ;
Hotchkiss, Arland T. .
FOOD HYDROCOLLOIDS, 2009, 23 (06) :1554-1562
1234