Cloning and characterization of the 14-3-3 protein gene from Ipomoea batatas (L.) Lam

被引：5

作者：

Chang, Mengjie ^{[1
]}

Tao, Xiang ^{[1
]}

Gu, Yinhong ^{[1
]}

Lin, Guoxiu ^{[1
]}

Shao, Huanhuan ^{[1
]}

Cao, Qinghua ^{[1
]}

Zhang, Yizheng ^{[1
]}

机构：

[1] Sichuan Univ, Coll Life Sci, Key Lab Resource Biol & Ecoenvironm, Minist Educ,Sichuan Key Lab Mol Biol & Biotechnol, Chengdu 610064, Peoples R China

来源：

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2012年 / 6卷 / 09期

关键词：

14-3-3; protein; Ipomoea batatas; gene cloning; digital gene expression profiling; RT-PCR analysis; DIFFERENTIAL EXPRESSION ANALYSIS; SWISS-MODEL; RNA-SEQ; ARABIDOPSIS; PLANT; 14-3-3-PROTEINS; ENVIRONMENT; REGULATORS; ISOFORM; STRESS;

D O I：

10.5897/AJMR11.1134

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The 14-3-3 proteins are a group of highly conserved and widely distributed eukaryotic proteins with diverse functions. In plants, it regulates basal metabolism, ion transport, cell passage, enzyme activity and gene expression, and it is also important in disease resistance, anti-oxidation and drought resistance. Based on assembled sequences of sweet potato (Ipomoea batatas L. Lam) transcriptome, 14-3-3 protein cDNAs were cloned for the first time, and the sequencing analysis revealed the presence of three highly homologous isoforms of 14-3-3 protein cDNAs, designated as Ib14-3-3a, Ib14-3-3b and Ib14-3-3c. Three cDNA isoforms are all 1176 bp in length with 7 mutation sites and have a 789 bp of open reading frame encoding a polypeptide of 262 amino acids. The results from digital gene expression (DGE) profiling showed that the expression levels of 14-3-3 genes are different among tissues, predominantly in harvested tuberous roots (223.08 TPM) and very low in mature leaves (85.07 TPM), which were also confirmed by semi-quantitative Reverse transcription polymerase chain reaction (RT-PCR) analysis.

引用

页码：1990 / 1999

页数：10

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