Label-free impedimetric aptasensor for lysozyme detection based on carbon nanotube-modified screen-printed electrodes

被引：107

作者：

Rohrbach, Falk ^{[2
]}

Karadeniz, Hakan ^{[1
]}

Erdem, Arzum ^{[1
]}

Famulok, Michael ^{[2
]}

Mayer, Guenter ^{[2
]}

机构：

[1] Ege Univ, Fac Pharm, Dept Analyt Chem, TR-35100 Izmir, Turkey

[2] Univ Bonn, Program Unit Chem Biol & Med Chem, LIMES Inst, D-53121 Bonn, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 421卷 / 02期

关键词：

Aptamer; Lysozyme; Filter retention assay; Electrochemical impedance spectroscopy; Carbon nanotube; Screen-printed electrodes; APTAMER-BASED BIOSENSOR; GRAPHITE-ELECTRODES; DNA HYBRIDIZATION; NUCLEIC-ACIDS; NANOPARTICLES; RECOGNITION; ENRICHMENT; SENSOR; TOOLS; BIND;

D O I：

10.1016/j.ab.2011.11.034

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the direct electrochemical detection of aptamer-protein interactions, namely between a DNA aptamer and lysozyme (LYS) based on electrochemical impedance spectroscopy (EIS) technique. First, the affinity of the aptamer to LYS and control proteins was presented by using filter retention assay. An amino-modified version of the DNA aptamer-recognizing lysozyme was covalently immobilized on the surface of multiwalled carbon nanotube-modified screen-printed electrodes (MWCNT-SPEs), which were employed for measurements and have improved properties compared with bare SPEs. This carbon nanotube setup enabled the reliable monitoring of the interaction of lysozyme with its cognate aptamer by EIS transduction of the resistance to charge transfer (R-ct) in the presence of 2.5 mM [Fe(CN)(6)](3-/4-). This assay system provides a means for the label-free, concentration-dependent, and selective detection of lysozyme with an observed detection limit of 12.09 mu g/ml (equal to 862 nM). (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：454 / 459

页数：6

共 37 条

[1] Impedimetric genosensing of DNA polymorphism correlated to cystic fibrosis: A comparison among different protocols and electrode surfaces [J].

Bonanni, Alessandra ;

Jose Esplandiu, Maria ;

del Valle, Manel .

BIOSENSORS & BIOELECTRONICS, 2010, 26 (04) :1245-1251

[2] A DNA-based machine that can cyclically bind and release thrombin [J].

Dittmer, WU ;

Reuter, A ;

Simmel, FC .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (27) :3550-3553

[3] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[4] Direct DNA hybridization at disposable graphite electrodes modified with carbon nanotubes [J].

Erdem, Arzum ;

Papakonstantinou, Pagona ;

Murphy, Hayley .

ANALYTICAL CHEMISTRY, 2006, 78 (18) :6656-6659

[5] Dendrimer modified graphite sensors for detection of anticancer drug Daunorubicin by voltammetry and electrochemical impedance spectroscopy [J].

Erdem, Arzum ;

Karadeniz, Hakan ;

Caliskan, Ayfer .

ANALYST, 2011, 136 (05) :1041-1045

[6] Streptavidin Modified Carbon Nanotube Based Graphite Electrode for Label-Free Sequence Specific DNA Detection [J].

Erdem, Arzum ;

Papakonstantinou, Pagona ;

Murphy, Hayley ;

McMullan, Martin ;

Karadeniz, Hakan ;

Sharma, Surbhi .

ELECTROANALYSIS, 2010, 22 (06) :611-617

[7] Electrochemical Sensing of Aptamer-Protein Interactions Using a Magnetic Particle Assay and Single-Use Sensor Technology [J].

Erdem, Arzum ;

Karadeniz, Hakan ;

Mayer, Guenter ;

Famulok, Michael ;

Caliskan, Ayfer .

ELECTROANALYSIS, 2009, 21 (11) :1278-1284

[8] Single-Walled Carbon Nanotubes Modified Graphite Electrodes for Electrochemical Monitoring of Nucleic Acids and Biomolecular Interactions [J].

Erdem, Arzum ;

Karadeniz, Hakan ;

Caliskan, Ayfer .

ELECTROANALYSIS, 2009, 21 (3-5) :464-471

[9]

Famulok M, 1999, CURR TOP MICROBIOL, V243, P123

[10] Functional aptamers and aptazymes in biotechnology, diagnostics, and therapy [J].

Famulok, Michael ;

Hartig, Jorg S. ;

Mayer, Gunter .

CHEMICAL REVIEWS, 2007, 107 (09) :3715-3743

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