Light-triggered C60 release from a graphene/cyclodextrin nanoplatform for the protection of cytotoxicity induced by nitric oxide

An ultraviolet (UV) light-triggered nanocarbon hybrid is developed for controlled C-60 release with excellent nitric oxide (NO) quenching ability. This nanocarrier, consisting of reduced graphene oxide (rGO) and beta-cyclodextrin (beta-CD), is capable of hosting azobenzene functionalized C-60 (Azo-C-60) synthesized by diazo chemistry. The hybridization of rGO, beta-CD and Azo-C-60 enhances cellular uptake and limits the aggregation of C-60, and shows enhanced protective effects on NO-induced cytotoxicity. More interestingly, azo groups can reversibly switch between trans-and cis-isomers upon UV irradiation, so that the Azo-C-60 molecules exhibit photo-controlled release from rGO/beta-CD in living cells. In vitro studies show that rGO/b-CD/C-60 treated with UV irradiation causes higher NO scavenging efficacy, which further significantly increases the cell viability from 32.6% to 88.4% at low loading levels (50 mg mL(-1)). This represents an excellent NO quenching efficiency, better than other reports of the graphene/C-60 nanohybrids, and indicates that this material can be an effective nanoplatform to combat oxidative damage. As the host-guest chemistry and diazo chemistry are versatile and universally applicable, it is worth noting that the present strategy can also be applied in preparing other photo-responsive nanohybrids, which should be valuable for use in life science and materials science.