miR-130b, an onco-miRNA in bladder cancer, is directly regulated by NF-κB and sustains NF-κB activation by decreasing Cylindromatosis expression

Persistent activation of NF-kappa B signaling is closely related to chronic inflammation and tumorgenesis. Commonly, NF-kappa B signaling is tightly controlled by multiple feedback loops and regulators, such as the deubiquitinases (DUBs). However, in cancer cells, NF-kappa B may override these feedbacks through special pathways and lead to the sustained activation. In the present study, we demonstrate that in transitional cell carcinoma (TCC) of bladder, miR-130b plays an oncogenesis role, it enhanced proliferation, invasion and migration of TCC cell, and was highly correlated with tumor progression. On the other hand, NF-kappa B directly regulated the transcription of miR-130b by binding with its promoter region. Importantly, we verify that, through deceasing the expression of Cylindromatosis (CYLD), a K63-specific DUB and endogenous blocker of NF-kappa B signaling, miR-130b can in return sustain the persistent activation of NF-kappa B, which may promote the malignant progression of TCC. Thus, the present study uncovers a potential signaling transduction in which NF-kappa B is continuously activated, and may provide a novel therapeutic approach for the clinical management of TCC.