Genome downsizing during ciliate development: Nuclear Division of Labor through chromosome restructuring

被引：110
作者：
Coyne, RS
Chalker, DL
Yao, MC
机构：
[1] Fred Hutchinson Cancer Res. Center, Seattle, WA 98104
来源：
ANNUAL REVIEW OF GENETICS | 1996年 / 30卷
关键词：
chromosome breakage; DNA deletion; ciliated protozoa; germline-soma differentiation; chromosome structure;
D O I：
10.1146/annurev.genet.30.1.557
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The ciliated protozoa divide the labor of germline and somatic genetic functions between two distinct nuclei. The development of the somatic (macro-) nucleus from the germinal (micro-) nucleus occurs during sexual reproduction and involves large-scale, genetic reorganization including site-specific chromosome breakage and DNA deletion. This intriguing process has been extensively studied in Tetrahymena thermophila. Characterization of cis-acting sequences, putative protein factors, and possible reaction intermediates has begun to shed light on the underlying mechanisms of genome rearrangement. This article summarizes the current understanding of this phenomenon and discusses its origin and biological function. We postulate that ciliate nuclear restructuring serves to segregate the two essential functions of chromosomes: the transmission and expression of genetic information.
引用
收藏
页码：557 / 578
页数：22
相关论文
共 109 条
[1]   MUTATIONS DEREPRESSING SILENT CENTROMERIC DOMAINS IN FISSION YEAST DISRUPT CHROMOSOME SEGREGATION [J].
ALLSHIRE, RC ;
NIMMO, ER ;
EKWALL, K ;
JAVERZAT, JP ;
CRANSTON, G .
GENES & DEVELOPMENT, 1995, 9 (02) :218-233
[2]   MACRONUCLEAR DNA OF TETRAHYMENA-THERMOPHILA EXISTS AS DEFINED SUB-CHROMOSOMAL-SIZED MOLECULES [J].
ALTSCHULER, MI ;
YAO, MC .
NUCLEIC ACIDS RESEARCH, 1985, 13 (16) :5817-5831
[3]   CHROMOSOME END FORMATION AND INTERNAL SEQUENCE ELIMINATION AS ALTERNATIVE GENOMIC REARRANGEMENTS IN THE CILIATE PARAMECIUM [J].
AMAR, L .
JOURNAL OF MOLECULAR BIOLOGY, 1994, 236 (02) :421-426
[4]   SEQUENCE STRUCTURES OF 2 DEVELOPMENTALLY REGULATED, ALTERNATIVE DNA DELETION JUNCTIONS IN TETRAHYMENA-THERMOPHILA [J].
AUSTERBERRY, CF ;
YAO, MC .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (09) :3947-3950
[5]  
AUSTERBERRY CF, 1984, P NATL ACAD SCI-BIOL, V81, P7383, DOI 10.1073/pnas.81.23.7383
[6]   SEQUENCE MICROHETEROGENEITY IS GENERATED AT JUNCTIONS OF PROGRAMMED DNA DELETIONS IN TETRAHYMENA-THERMOPHILA [J].
AUSTERBERRY, CF ;
SNYDER, RO ;
YAO, MC .
NUCLEIC ACIDS RESEARCH, 1989, 17 (18) :7263-7272
[7]   MICRONUCLEAR GENOME ORGANIZATION IN EUPLOTES-CRASSUS - A TRANSPOSON-LIKE ELEMENT IS REMOVED DURING MACRONUCLEAR DEVELOPMENT [J].
BAIRD, SE ;
FINO, GM ;
TAUSTA, SL ;
KLOBUTCHER, LA .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (09) :3793-3807
[8]   DIFFERENTIAL DNA AMPLIFICATION AND COPY NUMBER CONTROL IN THE HYPOTRICHOUS CILIATE EUPLOTES-CRASSUS [J].
BAIRD, SE ;
KLOBUTCHER, LA .
JOURNAL OF PROTOZOOLOGY, 1991, 38 (02) :136-140
[9]   CHARACTERIZATION OF CHROMOSOME FRAGMENTATION IN 2 PROTOZOANS AND IDENTIFICATION OF A CANDIDATE FRAGMENTATION SEQUENCE IN EUPLOTES-CRASSUS [J].
BAIRD, SE ;
KLOBUTCHER, LA .
GENES & DEVELOPMENT, 1989, 3 (05) :585-597
[10]   TELOMERIC SITE POSITION HETEROGENEITY IN MACRONUCLEAR DNA OF PARAMECIUM-PRIMAURELIA [J].
BAROIN, A ;
PRAT, A ;
CARON, F .
NUCLEIC ACIDS RESEARCH, 1987, 15 (04) :1717-1728
12345678910