Electroimmobilisation of sulphite oxidase into a polypyrrole film and its utilisation for flow amperometric detection of sulphite

被引：41
作者：
Adeloju, SB [1 ]
Barisci, JN [1 ]
Wallace, GG [1 ]
机构：
[1] UNIV WOLLONGONG,DEPT CHEM,INTELLIGENT POLYMER RES LAB,WOLLONGONG,NSW 2522,AUSTRALIA
来源：
ANALYTICA CHIMICA ACTA | 1996年 / 332卷 / 2-3期
基金：
澳大利亚研究理事会;
关键词：
sulphite oxidase; polypyrrole; immobilisation; characterisation; amperometry;
D O I：
10.1016/0003-2670(96)00261-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The immobilisation of sulphite oxidase into a polypyrrole him by galvanostatic polymerisation has been investigated. The properties of the film were characterised by radiolabelling technique, cyclic voltammetry, resistometry, electrochemical quartz crystal microbalance, scanning electron microscopy and atomic force microscopy. The radiolabelling measurements revealed that the amount of enzyme incorporated into the polypyrrole film is influenced by the enzyme concentration, film thickness and current density. The interaction of sulphite with the immobilised enzyme was confirmed by cyclic voltammetry, resistometry and electrochemical quartz crystal microbalance. Quantification of sulphite can be made from the amperometric response resulting from this interaction by flow injection analysis.
引用
收藏
页码：145 / 153
页数：9
相关论文
共 26 条
[1]   POLYPYRROLE-BASED POTENTIOMETRIC BIOSENSOR FOR UREA .2. ANALYTICAL OPTIMIZATION [J].
ADELOJU, SB ;
SHAW, SJ ;
WALLACE, GG .
ANALYTICA CHIMICA ACTA, 1993, 281 (03) :621-627
[2]   POLYPYRROLE-BASED POTENTIOMETRIC BIOSENSOR FOR UREA .1. INCORPORATION OF UREASE [J].
ADELOJU, SB ;
SHAW, SJ ;
WALLACE, GG .
ANALYTICA CHIMICA ACTA, 1993, 281 (03) :611-620
[3]   POLYPYRROLE-BASED AMPEROMETRIC BIOSENSOR FOR SULFITE DETERMINATION [J].
ADELOJU, SB ;
SHAW, SJ ;
WALLACE, GG .
ELECTROANALYSIS, 1994, 6 (10) :865-870
[4]  
BARISCI JN, UNPUB
[5]  
BARISCI JN, IN PRESS ELECTROANAL
[6]  
CHANG TMS, 1974, INSOLUBLE ENZYMES, P15
[7]  
COHEN HJ, 1971, J BIOL CHEM, V246, P359
[8]   AN ELECTROCHEMICALLY GROWN POLYMER AS AN IMMOBILIZATION MATRIX FOR WHOLE CELLS - APPLICATION IN AN AMPEROMETRIC DOPAMINE SENSOR [J].
DESHPANDE, MV ;
HALL, EAH .
BIOSENSORS & BIOELECTRONICS, 1990, 5 (06) :431-448
[9]   CHARACTERIZATION OF THE BIOCHEMICAL BEHAVIOR OF GLUCOSE-OXIDASE ENTRAPPED IN A POLYPYRROLE FILM [J].
FORTIER, G ;
BELANGER, D .
BIOTECHNOLOGY AND BIOENGINEERING, 1991, 37 (09) :854-858
[10]   OPTIMIZATION OF A POLYPYRROLE GLUCOSE-OXIDASE BIOSENSOR [J].
FORTIER, G ;
BRASSARD, E ;
BELANGER, D .
BIOSENSORS & BIOELECTRONICS, 1990, 5 (06) :473-490
123