Tomato transformation and transgenic plant production

被引：84

作者：

Cortina, C ^{[1
]}

Culiáñez-Macià, FA ^{[1
]}

机构：

[1] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2004年 / 76卷 / 03期

关键词：

acetosyringone; genetic transformation; Lycopersicon esculentum; tissue culture; transgenic plants; zeatin riboside;

D O I：

10.1023/B:TICU.0000009249.14051.77

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tomato transformation and regeneration were analysed and optimized. Cotyledon explants from Lycopersicon esculentum cv. UC82B, were infected by Agrobacterium tumefaciens strain LBA4404 harbouring the neomycin phosphotransferase (NPTII) reporter gene. The effects of phenolic compounds, vitamins and growth regulators on plant transformation and regeneration were studied. Increasing the vitamin thiamine concentration from 0.1 mg l(-1) in standard medium to 0.4 mg l(-1) decreased the chlorophyll lost that accompanied the expansion of necrotic areas in cotyledon explants. Optimal shoot regeneration rate was obtained with a balanced concentration of 0.5 mg l(-1) auxin indolelacetic acid (IAA) and 0.5 mg l(-1) cytokinin zeatin riboside. Finally, when the phenolic acetosyringone was present in the co-culture medium at 200 muM, confirmed transgenic lines reached 50% of antibiotic resistant shoots. Under the above conditions, the transformation efficiency reached 12.5%.

引用

页码：269 / 275

页数：7

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