Comparative study on the analytical performance of three waveforms for the determination of several aminoglycoside antibiotics with high performance liquid chromatography using amperometric detection

被引:31
作者
Cai, YQ
Cai, W
Cheng, J
Mou, SF
Lu, YQ
机构
[1] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
[2] Univ Sci & Technol Beijing, Dept Chem, Beijing 100083, Peoples R China
[3] Dionex Chem Corp, Sunnyvale, CA 94088 USA
关键词
waveforms; amperometric detection; ammoglycoside antibiotics; high performance liquid chromatography;
D O I
10.1016/j.chroma.2005.01.086
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A preliminary comparative study was carried out on the analytical performances of a new six-potential waveform and other two detection waveforms, triple-potential waveform and quadruple-potential waveform. The analytical performances compared included signal response, background noise, signal/noise ratio and signal stability. Compared with triple-potential waveform, and quadruple-potential waveform, the new six-potential waveform had higher signal response, signal/noise ratio, and sensitivity. As for determination reproducibility, the six-potential waveform also exhibited a slightly better performance than the other two waveforms. Under the selected experimental conditions based on the six-potential waveform, there is a linear correlation between peak area and concentration over two to three orders of magnitude for nine aminoglycoside antibiotics with a correlation coefficients better than 0.998 and the detection limits measured as three times the peak height signal-to-noise ratio for the nine aminoglycoside antibiotics were in the range of 0.0198-0.889 mu g/mL. The proposed method had been used to analyze real gentamicin sulphate drug sample. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:124 / 130
页数:7
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