Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence

被引：8

作者：

Xiong, Zhihang ^{[1
,2
]}

Wang, Hongjian

Li, Jiajin ^{[1
,3
]}

Liao, Ran ^{[1
,4
]}

Mai, Haoji ^{[2
]}

Guan, Caizhong ^{[2
]}

Guo, Zhiming ^{[1
]}

Yang, Shangpan ^{[2
]}

Chen, Yan ^{[1
]}

Liu, Biwang ^{[2
]}

Liu, Tong ^{[1
]}

Li, Hongyi ^{[2
]}

Ding, Wenzheng ^{[2
]}

Zeng, Yaguang ^{[2
]}

Ma, Hui ^{[4
,5
]}

机构：

[1] Tsinghua Univ, Shenzhen Int Grad Sch, Inst Ocean Engn, Shenzhen 518055, Peoples R China

[2] Foshan Univ, Dept Photoelect Technol, Foshan 528000, Peoples R China

[3] Tsinghua Univ, Dept Biomed Engn, Beijing 100084, Peoples R China

[4] Tsinghua Univ, Shenzhen Int Grad Sch, Guangdong Res Ctr Polarizat Imaging & Measurement, Shenzhen 518055, Peoples R China

[5] Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China

来源：

BIOSENSORS-BASEL | 2021年 / 11卷 / 11期

基金：

中国国家自然科学基金;

关键词：

polarized light scattering; fluorescence; suspended particles; EXTRACTION; ALGAE; SIZE;

D O I：

10.3390/bios11110416

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence, aiming to obtain an effective classification of microplastics and microalgae. Results show that the obtained classification accuracy is significantly higher than that for either of these two methods. The setup also successfully measures submicron particles and discriminates two species of Synechococcus. Our study demonstrates the feasibility of simultaneously measuring polarized light scattering and fluorescence, and the promising capability of our method for further aquatic environmental monitoring.

引用

页数：10

共 28 条

[1] Flow cytometry: basic principles and applications [J].