Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells

被引：0
作者：
Bulatova, Leisan [1 ]
Savenkova, Daria [1 ]
Nurgalieva, Alsina [1 ]
Reshetnikova, Daria [1 ]
Timonina, Arina [1 ]
Skripova, Vera [1 ]
Bogdanov, Mikhail [1 ,2 ]
Kiyamova, Ramziya [1 ]
机构：
[1] Kazan Fed Univ, Inst Fundamental Med & Biol, Res Lab Biomarker, Kazan, Russia
[2] Univ Texas Hlth Sci Ctr, McGovern Med Sch, Dept Biochem & Mol Biol, Houston, TX 77030 USA
来源：
FRONTIERS IN MOLECULAR BIOSCIENCES | 2022年 / 9卷
基金：
俄罗斯科学基金会;
关键词：
NaPi2b; MX35; antigen; topology; ECD; monoclonal antibody; ovarian cancer; PHOSPHATE TRANSPORTER NAPI2B; ANTIBODY-DRUG CONJUGATE; MONOCLONAL-ANTIBODIES; COTRANSPORTER; EPITOPE; PREDICTION; INSERTION; ANTIGEN; BINDING; DOMAIN;
D O I：
10.3389/fmolb.2022.895911
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
NaPi2b is a sodium-dependent phosphate transporter that belongs to the SLC34 family of transporters which is mainly responsible for phosphate homeostasis in humans. Although NaPi2b is widely expressed in normal tissues, its overexpression has been demonstrated in ovarian, lung, and other cancers. A valuable set of antibodies, including L2 (20/3) and MX35, and its humanized versions react strongly with an antigen on the surface of ovarian and other carcinoma cells. Although the topology of NaPi2b was predicted in silico, no direct experimental data are available for the orientation of NaPi2b extracellular domains in cancer cells. The presented results of antibody mapping of untagged NaPi2b in live ovarian carcinoma cells OVCAR-4 provide a platform for current and future epitope-based cancer therapies and serological diagnostics.
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页数：11
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共 58 条
  • [1] Intraperitoneal α-Particle Radioimmunotherapy of Ovarian Cancer Patients: Pharmacokinetics and Dosimetry of 211At-MX35 F(ab′)2-A Phase I Study
    Andersson, Hakan
    Cederkrantz, Elin
    Back, Tom
    Divgi, Chaitanya
    Elgqvist, Jorgen
    Himmelman, Jakob
    Horvath, Gyorgy
    Jacobsson, Lars
    Jensen, Holger
    Lindegren, Sture
    Palm, Stig
    Hultborn, Ragnar
    [J]. JOURNAL OF NUCLEAR MEDICINE, 2009, 50 (07) : 1153 - 1160
  • [2] Membrane topology of human ASBT (SLC10A2) determined by dual label epitope insertion scanning mutagenesis. New evidence for seven transmembrane domains
    Banerjee, A
    Swaan, PW
    [J]. BIOCHEMISTRY, 2006, 45 (03) : 943 - 953
  • [3] Oral peptide specific egg antibody to intestinal sodium-dependent phosphate co-transporter-2b is effective at altering phosphate transport in vitro and in vivo
    Bobeck, Elizabeth A.
    Hellestad, Erica M.
    Sand, Jordan M.
    Piccione, Michelle L.
    Bishop, Jeff W.
    Helvig, Christian
    Petkovich, Martin
    Cook, Mark E.
    [J]. POULTRY SCIENCE, 2015, 94 (06) : 1128 - 1137
  • [4] The Dolaflexin-based Antibody-Drug Conjugate XMT-1536 Targets the Solid Tumor Lineage Antigen SLC34A2/NaPi2b
    Bodyak, Natalya D.
    Mosher, Rebecca
    Yurkovetskiy, Aleksandr V.
    Yin, Mao
    Bu, Charlie
    Conlon, Patrick R.
    Demady, Damon R.
    Devit, Michael J.
    Gumerov, Dmitry R.
    Gurijala, Venu R.
    Lee, Winnie
    Mcgillicuddy, Dennis
    Park, Peter U.
    Poling, Laura L.
    Protopova, Marina
    Qin, Liuliang
    Stevenson, Cheri A.
    Ter-Ovanesyan, Elena
    Uttard, Alex
    Xiao, Dongmei
    Xu, Jian
    Xu, Ling
    Bergstrom, Donald A.
    Lowinger, Timothy B.
    [J]. MOLECULAR CANCER THERAPEUTICS, 2021, 20 (05) : 896 - 905
  • [5] Bogdanov M., 2018, FLIP FLOPPING MEMBRA, V1, P28, DOI [10.1007/978-3-319-43676-0_62-1, DOI 10.1007/978-3-319-43676-0_62-1]
  • [6] Bogdanov M, 2017, METHODS MOL BIOL, V1615, P105, DOI 10.1007/978-1-4939-7033-9_9
  • [7] Lipids and topological rules governing membrane protein assembly
    Bogdanov, Mikhail
    Dowhan, William
    Vitrac, Heidi
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2014, 1843 (08): : 1475 - 1488
  • [8] Structurally constrained tumor-specific epitope within the largest extracellular domain of sodium-dependent phosphate transporter NaPi2b
    Bulatova, L. F.
    Skripova, V.
    Nurgalieva, A.
    Reshetnikova, D.
    Savenkova, D. V.
    Timonina, A.
    Bogdanov, M. V.
    Kiyamova, R. G.
    [J]. ANNALS OF ONCOLOGY, 2021, 32 : S368 - S369
  • [9] THE PHYSIOLOGY OF PROTEIN S-ACYLATION
    Chamberlain, Luke H.
    Shipston, Michael J.
    [J]. PHYSIOLOGICAL REVIEWS, 2015, 95 (02) : 341 - 376
  • [10] Positive Charge Loading at Protein Termini Is Due to Membrane Protein Topology, Not a Translational Ramp
    Charneski, Catherine A.
    Hurst, Laurence D.
    [J]. MOLECULAR BIOLOGY AND EVOLUTION, 2014, 31 (01) : 70 - 84
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