Peptide-based targeting of fluorophores to peroxisomes in living cells

被引：13

作者：

Pap, EHW ^{[1
]}

Dansen, TB ^{[1
]}

Wirtz, KWA ^{[1
]}

机构：

[1] Univ Utrecht, Biochem Lipids Dept, NL-3508 TB Utrecht, Netherlands

来源：

TRENDS IN CELL BIOLOGY | 2001年 / 11卷 / 01期

关键词：

D O I：

10.1016/S0962-8924(00)01829-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Peptides carrying different fluorophores can be designed to incorporate spontaneously into living cells when added to the medium. By incorporating the peroxisome-targeting sequence PTS1, the peptide is recognized by the protein-import machinery of peroxisomes and, as a result, can accumulate in these organelles. Depending on the cell type, an inhibitor of the multidrug-resistance protein might be required to ensure strong accumulation. In this update, we discuss the potential of these peptide-linked fluorophores in solving issues related to organelle function and dynamics.

引用

页码：10 / 12

页数：3

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