New Insights for Diagnosis of Pineapple Fusariosis by MALDI-TOF MS Technique

被引:12
作者
Santos, Cledir [1 ]
Ventura, Jose Aires [2 ]
Lima, Nelson [3 ]
机构
[1] Univ La Frontera, Fac Sci & Engn, Dept Chem Sci & Nat Resources, CIBAMA,BIOREN UFRO, Ave Francisco Salazar 01145, Temuco 4811230, Chile
[2] INCAPER, Inst Capixaba Pesquisa Assistencia Tecn & Extensa, Vitoria, ES, Brazil
[3] Univ Minho, Micoteca Univ Minho, CEB Ctr Biol Engn, P-4710057 Braga, Portugal
关键词
FLIGHT MASS-SPECTROMETRY; IDENTIFICATION; TRICHODERMA; IDENTIFY; ICMS;
D O I
10.1007/s00284-016-1041-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Fusarium is one of the most economically important fungal genus, since it includes many pathogenic species which cause a wide range of plant diseases. Morphological or molecular biology identification of Fusarium species is a limiting step in the fast diagnosis and treatment of plant disease caused by these fungi. Mass spectrometry by matrix-assisted laser/desorption ionisation-time-of-flight (MALDI-TOF)-based fingerprinting approach was applied to the fungal growth monitoring and direct detection of strain Fusarium guttiforme E-480 inoculated in both pineapple cultivars P,rola and Imperial side shoots, that are susceptible and resistant, respectively, to this fungal strain. MALDI-TOF MS technique was capable to detect fungal molecular mass peaks in the susceptible pineapple stem side shoot tissue. It is assumed that these molecular masses are mainly constituted by ribosomal proteins. MALDI-TOF-based fingerprinting approach has herein been demonstrated to be sensitive and accurate for the direct detection of F. guttiforme E-480 molecular masses on both susceptible and resistant pineapple side stem free of any pre-treatment. According to the results obtained, the changing on molecular mass peaks of infected susceptible pineapple tissue together with the possibility of fungal molecular masses analysis into this pineapple tissue can be a good indication for an early diagnosis by MALDI-TOF MS of pineapple fusariosis.
引用
收藏
页码:206 / 213
页数:8
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