Isolation and characterization of microsatellite markers from the entomopathogenic fungus Metarhizium anisopliae

被引：40

作者：

Enkerli, J ^{[1
]}

Kölliker, R ^{[1
]}

Keller, S ^{[1
]}

Widmer, F ^{[1
]}

机构：

[1] Swiss Fed Res Stn Agroecol & Agr, CH-8046 Zurich, Switzerland

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 02期

关键词：

enriched library; genetic diversity; Metarhizium anisopliae; simple sequence repeat;

D O I：

10.1111/j.1471-8286.2005.00935.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fourteen polymorphic microsatellite markers were isolated from the entomopathogenic fungus, Metarhizium anisopliae, based on enriched genomic libraries. In order to assess allelic variability, the microsatellite loci were analysed in a collection of 34 isolates sampled from across Switzerland. The number of detected alleles in 14 loci ranged from two to eight and expected heterozygosity from 0.265 to 0.808. Because of the high expected heterozygosity, the 14 microsatellite loci are very useful for ecological studies and analysis of population diversity, and to identifying, monitoring, and tracking M. anisopliae strains applied as biological control agents.

引用

页码：384 / 386

页数：3

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