Lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon

被引：6

作者：

Gupta, Sonal ^{[1
,2
]}

Gupta, Nidhi ^{[3
]}

Tiwari, Pradeep ^{[1
,4
]}

Menon, Saji ^{[5
]}

Mathur, Praveen ^{[6
]}

Kothari, Shanker Lal ^{[2
]}

Nallapeta, Sivaramaiah ^{[5
]}

Medicherla, Krishna Mohan ^{[1
]}

Suravajhala, Prashanth ^{[1
]}

机构：

[1] Birla Inst Sci Res, Dept Biotechnol & Bioinformat, Jaipur 302021, Rajasthan, India

[2] Amity Univ Rajasthan, Dept Biotechnol, Jaipur 303002, Rajasthan, India

[3] IIS Univ, Dept Biotechnol, Jaipur 302020, Rajasthan, India

[4] Manipal Univ, Sch Basic Sci, Dept Chem, Jaipur 303007, Rajasthan, India

[5] World Trade Ctr, NanoTemper Technol, Bengaluru 302017, India

[6] SMS Med Coll & Hosp, Dept Paediat Surg, JLN Marg, Jaipur 302004, Rajasthan, India

来源：

BIOMOLECULES | 2018年 / 8卷 / 03期

关键词：

long non coding RNA; whole exome sequencing; protein interaction; congenital pouch colon; microscale thermophoresis; LONG NONCODING RNAS; MODIFIER GENES; DISEASE; GENETICS; LNCRNA;

D O I：

10.3390/biom8030095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Congenital Pouch Colon (CPC) is a rare anorectal anomaly common to northwestern India, specifically Rajasthan. Despite efforts to understand the clinical genetic makeup of CPC, no attempt on identifying non-coding RNAs was done. We have earlier reported CPC's rare variants from whole exome sequencing (WES) across 18 affected samples in a total of 64 subjects. A Smith-Waterman algorithm was used to infer a couple of lncRNAs from WES samples of CPC with predictions from the Noncode database. Further screening and quantification using polymerase chain reaction (PCR), we ascertained interactions using Micro Scale Thermophoresis (MST). We report the role of lnc-EPB41-1-1 shown to be promiscuously interacting with KIF13A substantiating their role in regulation.

引用

页数：9

共 27 条

[21] Potential role of IncRNA cyp2c91-protein interactions on disease of the immune system [J].