Neodymium oxide nanostructures and their cytotoxic evaluation in human cancer cells

被引:8
作者
Ahmad, Javed [1 ]
Wahab, Rizwan [1 ,2 ]
Siddiqui, Maqsood A. [1 ]
Farshori, Nida Nayyar [3 ]
Saquib, Quaiser [1 ,2 ]
Ahmad, Naushad [4 ]
Al-Khedhairy, Abdulaziz A. [1 ,2 ]
机构
[1] King Saud Univ, Coll Sci, Chair DNA Res, Zool Dept, Riyadh 11451, Saudi Arabia
[2] King Saud Univ, Coll Sci, Zool Dept, Riyadh 11451, Saudi Arabia
[3] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11495, Saudi Arabia
[4] King Saud Univ, Coll Sci, Dept Chem, Riyadh 11451, Saudi Arabia
关键词
Neodymium oxides; TEM; MTT; NRU; ROS; Cell lines; RARE-EARTH-ELEMENTS; ND2O3; NANOPARTICLES; PERMANENT-MAGNETS; NEUTRAL RED; IN-VITRO; TOXICITY; ENHANCEMENT; PERFORMANCE; EXPOSURE; HEALTH;
D O I
10.1016/j.jtemb.2022.127029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neodymium oxide exhibits a unique property, which facilitates and largely utilized as an industrial applications. A number of cytotoxic study is available but very limited information is available to understand their biological activity with neodymium oxide at a very low conc-entration of the material. The present work was designed to understand the cytotoxicity against liver (HepG-2) and lung (A-549) cancer cells. Initially, Neodymium oxides (Nd2O3) were prepared and characterized with various instruments. The crystallinity and morphology of Nd2O3 powder were examined with instruments such as X-Ray Diffraction (XRD), scanning electron microscope (SEM), Transmission electron microscopy (TEM), Energy Dispersive X-Ray Analysis (EDX) respectively, revealed the size of curved nanostructure are-140 & PLUSMN; 2 in diameter whereas length goes upto-700 nm with elemental composition. The cytotoxicity study was conducted with MTT, NRU assay with genotoxicity study via ROS, cell cycle and qPCR analysis. The cells cytotoxic assessment were analysed via MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl tetra zolium Bromide) and Neutral Red Uptake (NRU) assay with neodymium oxide (Nd2O3), which indicates the reduction in cell viability. Additionally, cell-cycle analysis showed an increase in the apoptotic peak after a 24-h. Quantitative real-time PCR (RT-PCR) data revealed that apoptotic genes such as p53, bax, and caspase-3 were up regulated, whereas bcl-2, an anti-apoptotic gene, was down regulated; therefore, apoptosis was mediated through ROS and genotoxicity pathways. The experiments of cytotoxicity was tested and concludes that the Nd2O3 express a moderate and dose dependent effect on cancer cells. The ROS, cell cycle analysis and qPCR showed that Nd2O3 exhibit the capability to cells death via ROS generation and genotoxicity study pathways.
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页数:10
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