Titanium implants induce expression of matrix metalloproteinases in bone during osseointegration

被引:20
作者
Shubayev, VI
Brånemark, R
Steinauer, J
Myers, RR
机构
[1] Univ Calif San Diego, Dept Anesthesiol 0629, Sch Med, La Jolla, CA 92093 USA
[2] Inst Appl Biotechnol, Gothenburg, Sweden
[3] San Diego Dept Vet Affairs Healthcare Syst, San Diego, CA USA
关键词
bone; endosteal; histopathology; intramedullary; matrix; MMP; osseointegration; TIMP; titanium; TNF;
D O I
10.1682/JRRD.2003.07.0107
中图分类号
R49 [康复医学];
学科分类号
100215 ;
摘要
Implanted pure titanium fixtures are able to completely integrate with bone, in part because of the fort-nation of a strong extracellular matrix (ECM) bond at the titanium-bone interface. In this study, we used a rodent femur model of intramedullary osseointegration to analyze the changes in immunoreactivity of ECM-controlling matrix metal loprotelnases (MMPs), tissue inhibitor of metalloproteinase-3 (TIMP-3), and tumor necrosis factor alpha (TNF-alpha) during osseointegration. We observed dramatic increases in MMP-2, NIMP-9, MMP-7, TIMP-3, and TNF-alpha in osteocytes, osteoclasts, haversian canals, and the interface matrix in bone ipsilateral to the titanium implant. An increase in TIMP-3, NIMP-9, and MMP-7 in hypertrophied chondrocytes and the vascular component of the epiphysial growth plate was also observed in experimental bone. These findings were not seen in contralateral or sham-operated bone, where the titanium fixtures were threaded into the femur and immediately removed. Our data link titanium-induced bone remodeling, to changes in expression and distribution of MMPs.
引用
收藏
页码:757 / 765
页数:9
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