NanoFlares for the detection, isolation, and culture of live tumor cells from human blood

被引：178
作者：
Halo, Tiffany L. [1 ,3 ]
McMahon, Kaylin M. [4 ,6 ,7 ]
Angeloni, Nicholas L. [4 ,5 ,6 ]
Xu, Yilin [4 ,9 ]
Wang, Wei [4 ,9 ]
Chinen, Alyssa B. [1 ,3 ]
Malin, Dmitry [8 ]
Strekalova, Elena [8 ]
Cryns, Vincent L. [8 ]
Cheng, Chonghui [4 ,9 ]
Mirkin, Chad A. [1 ,2 ,3 ]
Thaxton, C. Shad [3 ,4 ,5 ,6 ]
机构：
[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
[2] Northwestern Univ, Dept Mat Sci & Engn, Evanston, IL 60208 USA
[3] Northwestern Univ, Int Inst Nanotechnol, Evanston, IL 60208 USA
[4] Northwestern Univ, Robert H Lurie Comprehens Canc Ctr, Chicago, IL 60611 USA
[5] Northwestern Univ, Feinberg Sch Med, Dept Urol, Chicago, IL 60611 USA
[6] Northwestern Univ, Simpson Querrey Inst BioNanotechnol Med, Chicago, IL 60611 USA
[7] Northwestern Univ, Walter S & Lucienne Driskill Grad Training Progra, Chicago, IL 60611 USA
[8] Univ Wisconsin, Sch Med & Publ Hlth, Carbone Canc Ctr, Div Endocrinol Diabet & Metab,Dept Med, Madison, WI 53705 USA
[9] Northwestern Univ, Feinberg Sch Med, Dept Med, Div Hematol Oncol, Chicago, IL 60611 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 48期
关键词：
cancer metastasis; nanotechnology; diagnostic; mRNA; NanoFlares; METASTATIC BREAST-CANCER; EPITHELIAL-MESENCHYMAL TRANSITION; MESSENGER-RNA DETECTION; PERIPHERAL-BLOOD; PROGRESSION; REGULATOR; MECHANISM; SURVIVAL; PREDICT;
D O I：
10.1073/pnas.1418637111
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Metastasis portends a poor prognosis for cancer patients. Primary tumor cells disseminate through the bloodstream before the appearance of detectable metastatic lesions. The analysis of cancer cells in blood-so-called circulating tumor cells (CTCs)-may provide unprecedented opportunities for metastatic risk assessment and investigation. NanoFlares are nanoconstructs that enable livecell detection of intracellular mRNA. NanoFlares, when coupled with flow cytometry, can be used to fluorescently detect genetic markers of CTCs in the context of whole blood. They allow one to detect as few as 100 live cancer cells per mL of blood and subsequently culture those cells. This technique can also be used to detect CTCs in a murine model of metastatic breast cancer. As such, NanoFlares provide, to our knowledge, the first genetic-based approach for detecting, isolating, and characterizing live cancer cells from blood and may provide new opportunities for cancer diagnosis, prognosis, and personalized therapy.
引用
收藏
页码：17104 / 17109
页数：6
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