NanoFlares for the detection, isolation, and culture of live tumor cells from human blood

被引：178

作者：

Halo, Tiffany L. ^{[1
,3
]}

McMahon, Kaylin M. ^{[4
,6
,7
]}

Angeloni, Nicholas L. ^{[4
,5
,6
]}

Xu, Yilin ^{[4
,9
]}

Wang, Wei ^{[4
,9
]}

Chinen, Alyssa B. ^{[1
,3
]}

Malin, Dmitry ^{[8
]}

Strekalova, Elena ^{[8
]}

Cryns, Vincent L. ^{[8
]}

Cheng, Chonghui ^{[4
,9
]}

Mirkin, Chad A. ^{[1
,2
,3
]}

Thaxton, C. Shad ^{[3
,4
,5
,6
]}

机构：

[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA

[2] Northwestern Univ, Dept Mat Sci & Engn, Evanston, IL 60208 USA

[3] Northwestern Univ, Int Inst Nanotechnol, Evanston, IL 60208 USA

[4] Northwestern Univ, Robert H Lurie Comprehens Canc Ctr, Chicago, IL 60611 USA

[5] Northwestern Univ, Feinberg Sch Med, Dept Urol, Chicago, IL 60611 USA

[6] Northwestern Univ, Simpson Querrey Inst BioNanotechnol Med, Chicago, IL 60611 USA

[7] Northwestern Univ, Walter S & Lucienne Driskill Grad Training Progra, Chicago, IL 60611 USA

[8] Univ Wisconsin, Sch Med & Publ Hlth, Carbone Canc Ctr, Div Endocrinol Diabet & Metab,Dept Med, Madison, WI 53705 USA

[9] Northwestern Univ, Feinberg Sch Med, Dept Med, Div Hematol Oncol, Chicago, IL 60611 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 48期

关键词：

cancer metastasis; nanotechnology; diagnostic; mRNA; NanoFlares; METASTATIC BREAST-CANCER; EPITHELIAL-MESENCHYMAL TRANSITION; MESSENGER-RNA DETECTION; PERIPHERAL-BLOOD; PROGRESSION; REGULATOR; MECHANISM; SURVIVAL; PREDICT;

D O I：

10.1073/pnas.1418637111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Metastasis portends a poor prognosis for cancer patients. Primary tumor cells disseminate through the bloodstream before the appearance of detectable metastatic lesions. The analysis of cancer cells in blood-so-called circulating tumor cells (CTCs)-may provide unprecedented opportunities for metastatic risk assessment and investigation. NanoFlares are nanoconstructs that enable livecell detection of intracellular mRNA. NanoFlares, when coupled with flow cytometry, can be used to fluorescently detect genetic markers of CTCs in the context of whole blood. They allow one to detect as few as 100 live cancer cells per mL of blood and subsequently culture those cells. This technique can also be used to detect CTCs in a murine model of metastatic breast cancer. As such, NanoFlares provide, to our knowledge, the first genetic-based approach for detecting, isolating, and characterizing live cancer cells from blood and may provide new opportunities for cancer diagnosis, prognosis, and personalized therapy.

引用

页码：17104 / 17109

页数：6

共 50 条

[21] Implementing microwell slides for detection and isolation of single circulating tumor cells from complex cell suspensions
Yang, Liwen
Rivandi, Mahdi
Franken, Andre
Hieltjes, Maarten
van der Zaag, Pieter Jan
Nelep, Constantin
Eberhardt, Jens
Peter, Stefan
Niederacher, Dieter
Fehm, Tanja
Neubauer, Hans
CYTOMETRY PART A, 2022, 101 (12) : 1057 - 1067
[22] Detection of circulating tumor cells in peripheral blood of heavily treated metastatic breast cancer patients
Tokudome, Nahomi
Ito, Yoshinori
Takahashi, Shunji
Kobayashi, Kokoro
Taira, Shinichiro
Tsutsumi, Chizuko
Oto, Masafumi
Oba, Masaru
Inoue, Kenichi
Kuwayama, Akiko
Masumura, Kyoko
Nakayama, Yoshie
Watanabe, Chie
Hatake, Kiyohiko
BREAST CANCER, 2011, 18 (03) : 195 - 202
[23] Isolation and characterization of circulating tumor cells from human gastric cancer patients
Yuan, Dandan
Chen, Liang
Li, Mingxing
Xia, Hongwei
Zhang, Yuchen
Chen, Tie
Xia, Rui
Tang, Qiulin
Gao, Fabao
Mo, Xianming
Liu, Ming
Bi, Feng
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2015, 141 (04) : 647 - 660
[24] Microfluidic Isolation of Circulating Tumor Cells and Cancer Stem-Like Cells from Patients with Pancreatic Ductal Adenocarcinoma
Varillas, Jose I.
Zhang, Jinling
Chen, Kangfu
Barnes, Isis I.
Liu, Chen
George, Thomas J.
Fan, Z. Hugh
THERANOSTICS, 2019, 9 (05): : 1417 - 1425
[25] Characterization of a novel automated microfiltration device for the efficient isolation and analysis of circulating tumor cells from clinical blood samples
Yee-de Leon, Juan F.
Soto-Garcia, Brenda
Araiz-Hernandez, Diana
Rolando Delgado-Balderas, Jesus
Esparza, Miguel
Aguilar-Avelar, Carlos
Wong-Campos, J. D.
Chacon, Franco
Lopez-Hernandez, Josey
Mauricio Gonzalez-Trevino, A.
Yee-de Leon, Jose R.
Zamora-Mendoza, Jorge L.
Alvarez, Mario M.
Trujillo-de Santiago, Grissel
Gomez-Guerra, Lauro S.
Sanchez-Dominguez, Celia N.
Velarde-Calvillo, Liza P.
Abarca-Blanco, Alejandro
SCIENTIFIC REPORTS, 2020, 10 (01)
[26] Ambient mass spectrometry-based detection system for tumor cells in human blood
Sakamoto, Kaname
Fujita, Yuichiro
Chikamatsu, Kazuaki
Tanaka, Shota
Takeda, Sen
Masuyama, Keisuke
Yoshimura, Kentaro
Ishii, Hiroki
TRANSLATIONAL CANCER RESEARCH, 2018, 7 (03) : 758 - 764
[27] Multicolor Detection of Rare Tumor Cells in Blood Using a Novel Flow Cytometry-Based System
Watanabe, Masaru
Uehara, Yuri
Yamashita, Namiko
Fujimura, Yuu
Nishio, Kaori
Sawada, Takeshi
Takeda, Kazuo
Koizumi, Fumiaki
Koh, Yasuhiro
CYTOMETRY PART A, 2014, 85 (03) : 206 - 213
[28] Nanomaterial-Based Immunocapture Platforms for the Recognition, Isolation, and Detection of Circulating Tumor Cells
Liu, Yichao
Li, Rui
Zhang, Lingling
Guo, Shishang
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2022, 10
[29] Isolation of circulating tumor cells from pancreatic cancer by automated filtration
Brychta, Nora
Drosch, Michael
Driemel, Christiane
Fischer, Johannes C.
Neves, Rui P.
Esposito, Irene
Knoefel, Wolfram
Moehlendick, Birte
Hille, Claudia
Stresemann, Antje
Krahn, Thomas
Kassack, Matthias U.
Stoecklein, Nikolas H.
von Ahsen, Oliver
ONCOTARGET, 2017, 8 (49) : 86143 - 86156
[30] Acoustofluidic, Label-Free Separation and Simultaneous Concentration of Rare Tumor Cells from White Blood Cells
Antfolk, Maria
Magnusson, Cecilia
Augustsson, Per
Lija, Hans
Laurell, Thomas
ANALYTICAL CHEMISTRY, 2015, 87 (18) : 9322 - 9328

← 1 2 3 4 5 →