Using a deoxyribozyme ligase and rolling circle amplification to detect a non-nucleic acid analyte, ATP

被引：172

作者：

Cho, EJ ^{[1
]}

Yang, LT ^{[1
]}

Levy, M ^{[1
]}

Ellington, AD ^{[1
]}

机构：

[1] Univ Texas, Inst Cell & Mol Biol, Dept Chem & Biochem, Austin, TX 78712 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 07期

关键词：

D O I：

10.1021/ja043490u

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An allosteric ribozyme (aptazyme) has been used to transduce the binding of a small organic analyte (ATP) into the ligation of a circular template for rolling circle amplification (RCA). An ATP-activated deoxyribozyme ligase was immobilized on a glass slide and, upon addition of ATP, catalyzed the ligation of a circular padlock probe. The ligated products could be directly amplified and visualized via RCA. The coupled reaction exhibited could detect as little as 1 μM of ATP and could discriminate against structurally similar nucleotides such as GTP, CTP, and UTP. Cooperative ATP activation of the deoxyribozyme was faithfully mimicked by RCA, yielding an amplified switch" that was responsive to ATP concentration. Copyright © 2005 American Chemical Society."

引用

页码：2022 / 2023

页数：2

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