Cholesterol esterase activity of a molecularly imprinted polymer

被引：1
|
作者：
Kim, JM [1 ]
Ahn, KD
Wulff, G
机构：
[1] Univ Dusseldorf, Inst Organ Chem & Macromol Chem, D-40225 Dusseldorf, Germany
[2] Korea Inst Sci & Technol, Funct Polymer Lab, Div Life Sci, Seoul 130650, South Korea
来源：
MACROMOLECULAR CHEMISTRY AND PHYSICS | 2001年 / 202卷 / 07期
关键词：
D O I：
10.1002/1521-3935(20010401)202:7<1105::AID-MACP1105>3.0.CO;2-P
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
A molecular imprinted polymer with cholesterol esterase activity was prepared. Polymerization of N,N′-diethyl(4-vinylphenyl)amidine in the presence of the phosphate transition state analogue 2 followed by removal of the print molecule generated a catalytically active polymer with Km of 3.7 mM and Kcat of 2.2·10-4 min-1, respectively. A relative rate enhancement of 27-fold in the presence of the control polymer were observed.
引用
收藏
页码：1105 / 1108
页数：4
相关论文
共 50 条
  • [21] Molecularly imprinted anisotropic polymer monoliths
    Steinke, JHG
    Dunkin, IR
    Sherrington, DC
    MACROMOLECULES, 1996, 29 (01) : 407 - 415
  • [22] Molecularly Imprinted Polymer for Separation of Lactate
    Divya, M. P.
    Rajput, Y. S.
    Sharma, R.
    Singh, G.
    JOURNAL OF ANALYTICAL CHEMISTRY, 2015, 70 (10) : 1213 - 1217
  • [23] Janus molecularly imprinted polymer particles
    Huang, Chuixiu
    Shen, Xiantao
    CHEMICAL COMMUNICATIONS, 2014, 50 (20) : 2646 - 2649
  • [24] Which molecularly imprinted polymer is better?
    Toth, B.
    Pap, T.
    Horvath, V.
    Horvai, G.
    ANALYTICA CHIMICA ACTA, 2007, 591 (01) : 17 - 21
  • [25] Molecularly imprinted polymer sensor arrays
    Greene, NT
    Morgan, SL
    Shimizu, KD
    CHEMICAL COMMUNICATIONS, 2004, (10) : 1172 - 1173
  • [26] Molecularly imprinted polymer sensor arrays
    Shimizu, Ken D.
    Stephenson, Clifton J.
    CURRENT OPINION IN CHEMICAL BIOLOGY, 2010, 14 (06) : 743 - 750
  • [27] A molecularly imprinted polymer for the determination of neopterin
    Del Sole, Roberta
    Scardino, Anna
    Lazzoi, Maria Rosaria
    Mergola, Lucia
    Scorrano, Sonia
    Vasapollo, Giuseppe
    MICROCHIMICA ACTA, 2013, 180 (15-16) : 1401 - 1409
  • [28] A molecularly imprinted polymer for the pesticide bentazone
    Baggiani, C
    Trotta, F
    Giraudi, G
    Giovannoli, C
    Vanni, A
    ANALYTICAL COMMUNICATIONS, 1999, 36 (07): : 263 - 266
  • [29] Synthesis of a molecularly imprinted polymer for dioxin
    Malitesta, Cosimino
    Picca, Rosaria Anna
    Ciccarella, Giuseppe
    Sgobba, Vito
    Brattoli, Magda
    SENSORS, 2006, 6 (08) : 915 - 924
  • [30] Fabrication of molecularly imprinted polymer microstructures
    Yan, M
    Kapua, A
    ANALYTICA CHIMICA ACTA, 2001, 435 (01) : 163 - 167
12345